Department of Chemistry, University of Southern California, Los Angeles, CA 90089, USA.
Bioorg Med Chem Lett. 2011 Sep 1;21(17):5062-6. doi: 10.1016/j.bmcl.2011.04.038. Epub 2011 Apr 14.
Mucin-type O-linked glycosylation is a common post translational modification of cell-surface and secretory pathway proteins and is implicated in vital biological processes as well as human disease. We report here the use of the metabolic chemical reporter GalNAz along with Cu(I)-catalyzed [3+2] azide-alkyne cycloaddition conditions for the robust, in-gel fluorescent visualization of mucin-type O-linked glycoproteins.
黏蛋白型 O-糖基化是一种常见的细胞表面和分泌途径蛋白的翻译后修饰，与重要的生物过程以及人类疾病有关。我们在这里报告了使用代谢化学报告物 GalNAz 以及 Cu(I) 催化的 [3+2] 叠氮-炔环加成条件，用于稳健的、凝胶内荧光可视化黏蛋白型 O-糖蛋白。
