Chu Fengting, Feng Qiping, Qian Yufen, Zhang Chunye, Fang Zhengyu, Shen Gang
Department of Orthodontics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, China.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011 Jul;112(1):90-5. doi: 10.1016/j.tripleo.2011.01.026. Epub 2011 Apr 30.
Chromosomal instability is hallmark of carcinoma. Amplification of chromosome 17q11-q12 is present in some oral squamous cell cancer (OSCC) cases. In this study, we investigated the copy number variations of ERBB2 gene, which is located at this locus in collected OSCC samples and their correlation with tumor progression and gene expression.
Quantitative real-time polymerase chain reaction was performed to detect the copy number of ERBB2 gene and the mRNA expression in 92 OSCC samples with matched adjacent normal tissues (ANTs). Proportional odds regression and 2-way repeated measurement analysis of variance were used to analyze the association between copy number variations and mRNA expression of the targeted gene.
Copy number gains of ERBB2 were detected in some of the OSCCs (19.6%, 18/92) and correlated with tumor stage (P < .001). Copy number gains of ERBB2 also showed a positive correlation with mRNA overexpression in OSCCs (P < .001). However, enhanced ERBB2 mRNA expression was also detected in a group of OSCC samples with unaltered copy number of ERBB2 gene (P < .05).
Copy number increase of ERBB2 is observed in OSCCs and correlates with gene overexpression in these tumors. In addition, overexpression of ERBB2 is also observed in some OSCCs that lack copy number changes, indicating involvement of another mechanism.
染色体不稳定是癌症的标志。17q11 - q12染色体扩增存在于部分口腔鳞状细胞癌（OSCC）病例中。在本研究中，我们调查了位于该位点的ERBB2基因在收集的OSCC样本中的拷贝数变异及其与肿瘤进展和基因表达的相关性。
采用定量实时聚合酶链反应检测92例OSCC样本及其匹配的癌旁正常组织（ANT）中ERBB2基因的拷贝数和mRNA表达。使用比例优势回归和双向重复测量方差分析来分析目标基因拷贝数变异与mRNA表达之间的关联。
在部分OSCC样本中检测到ERBB2基因拷贝数增加（19.6%，18/92），且与肿瘤分期相关（P <.001）。ERBB2基因拷贝数增加在OSCC中也与mRNA过表达呈正相关（P <.001）。然而，在一组ERBB2基因拷贝数未改变的OSCC样本中也检测到ERBB2 mRNA表达增强（P <.05）。
在OSCC中观察到ERBB2基因拷贝数增加，且与这些肿瘤中的基因过表达相关。此外，在一些缺乏拷贝数变化的OSCC中也观察到ERBB2过表达，表明存在另一种机制。
