Miller M D, Lord C I, Stallard V, Mazzara G P, Letvin N L
Harvard Medical School, New England Regional Primate Research Center, Southborough, MA 01772.
J Immunol. 1990 Jan 1;144(1):122-8.
The simian immunodeficiency virus of macaques (SIVmac) is a lentivirus which induces an AIDS-like disease in rhesus monkeys. We have explored the virus-specific cellular immune response in SIVmac-infected rhesus monkeys. Con A-activated, IL-2 expanded PBL of some SIVmac-infected rhesus monkeys lyse autologous B lymphoblastoid cell lines infected with a recombinant vaccinia virus that carries the SIVmac gag gene. This lysis is mediated by CD8+ lymphocytes and is MHC class I restricted. Moreover, these effector lymphocytes do not express the NK cell-associated molecules NKH1 or CD16. These cells are, therefore, CTL. In a limited prospective study of SIVmac-infected rhesus monkeys, the presence of the SIVmac gag-specific CTL activity in PBL correlated with both a reduced efficiency in isolating SIVmac from PBL of these monkeys and their extended survival. This method for assessing SIVmac gag-specific cellular immunity in rhesus monkeys will be important not only in investigating the immunopathogenesis of SIVmac-induced disease, but also in evaluating the capacity of candidate AIDS vaccines to elicit a cell-mediated immune response in this animal model.
猕猴猿猴免疫缺陷病毒（SIVmac）是一种慢病毒，可在恒河猴中引发类似艾滋病的疾病。我们探究了SIVmac感染的恒河猴体内病毒特异性细胞免疫反应。一些SIVmac感染的恒河猴经刀豆蛋白A激活、白细胞介素-2扩增的外周血淋巴细胞（PBL）可裂解被携带SIVmac gag基因的重组痘苗病毒感染的自体B淋巴母细胞系。这种裂解由CD8 +淋巴细胞介导，且受MHC I类分子限制。此外，这些效应淋巴细胞不表达自然杀伤细胞相关分子NKH1或CD16。因此，这些细胞是细胞毒性T淋巴细胞（CTL）。在一项对SIVmac感染的恒河猴的有限前瞻性研究中，PBL中SIVmac gag特异性CTL活性的存在与从这些猴子的PBL中分离SIVmac的效率降低及其生存期延长均相关。这种评估恒河猴中SIVmac gag特异性细胞免疫的方法不仅对于研究SIVmac诱导疾病的免疫发病机制很重要，而且对于评估候选艾滋病疫苗在该动物模型中引发细胞介导免疫反应的能力也很重要。
