Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan.
J Biochem. 2011 Sep;150(3):327-39. doi: 10.1093/jb/mvr052. Epub 2011 Apr 29.
Alkylation is a type of stress that is fatal to cells. However, cells have various responses to alkylation. Alkyltransferase-like (ATL) protein is a novel protein involved in the repair of alkylated DNA; however, its repair mechanism at the molecular level is unclear. DNA microarray analysis revealed that the upregulation of 71 genes because of treatment with an alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine was related to the presence of TTHA1564, the ATL protein from Thermus thermophilus HB8. Affinity chromatography showed a direct interaction of purified TTHA1564 with purified RNA polymerase holoenzyme. The amino acid sequence of TTHA1564 is homologous to that of the C-terminal domain of Ada protein, which acts as a transcriptional activator. These results suggest that TTHA1564 might act as a transcriptional regulator. The results of DNA microarray analysis also implied that the alkylating agent induced oxidation stress in addition to alkylation stress.
烷基化是一种对细胞致命的应激类型。然而，细胞对烷基化有各种反应。烷基转移酶样（ATL）蛋白是一种涉及修复烷基化 DNA 的新型蛋白质；然而，其在分子水平上的修复机制尚不清楚。DNA 微阵列分析显示，由于用烷化剂 N-甲基-N'-硝基-N-亚硝基胍处理，71 个基因的上调与 Thermus thermophilus HB8 的 ATL 蛋白 TTHA1564 的存在有关。亲和层析显示纯化的 TTHA1564 与纯化的 RNA 聚合酶全酶直接相互作用。TTHA1564 的氨基酸序列与作为转录激活物的 Ada 蛋白 C 末端结构域的氨基酸序列同源。这些结果表明 TTHA1564 可能作为转录调节剂发挥作用。DNA 微阵列分析的结果还表明，烷化剂除了诱导烷基化应激外，还诱导氧化应激。
