Institute of Biotechnology, Hoang Quoc Viet Rd, Cau Giay, Hanoi, Vietnam.
J Microbiol Biotechnol. 2011 Apr;21(4):405-11.
Avian influenza virus vaccines produced in oil-emulsified inactivated form with antigen content of at least 160 hemagglutinin units (HAU) induced immunity in birds. However, in addition to enhancing the effect of the adjuvant(s), other additional supplemented biological compounds included in inactivated vaccines could produce higher levels of antibody. We examined in chickens, Vietnamese ducks, and muscovy ducks the adjuvant effect of Sophy β-glucan (SBG), a β-1,3-1,6 glucan produced by the black yeast Aureobasidium pollulans strain AF0-202, when administered with an avian influenza H5 subtype vaccine. In Experiment 1, 40 chickens (ISA Brown hybrid), allocated to four groups of ten each, were immunized with Oil-H5N1(VN), Oil-H5N1(CN), Oil-H5N2(CN), and saline (control group), respectively. In Experiment 2, chickens (ISA Brown hybrid), muscovy ducks (French hybrid), and Vietnamese ducks (indigenous Vietnamese) were used to further assess the effect of SBG on immunogenicity of the Oil-H5N1(VN) Vietnamese vaccine. ELISA and hemagglutination inhibition (HI) assays were used to assess the antibody response. The H5 subtype vaccines initiated significantly higher immune responses in the animals dosed with SBG, with 1.0-1.5 log2 higher HI titers and 10-20% ELISA seroconversion, compared with those not dosed with β-glucan. Notably, some of the animals dosed with SBG induced HI titers higher than 9.0 log2 following boosting immunization. Taken together, our serial studies indicated that SBG is a potential effector, such as enhancing the immune response to the H5 vaccines tested.
禽流感病毒疫苗以油包水型乳化剂制成，抗原含量至少为 160 个血凝素单位（HAU），可诱导禽类产生免疫。然而，除了增强佐剂的效果外，其他额外添加的生物化合物也包含在灭活疫苗中，可能会产生更高水平的抗体。我们在鸡、越南鸭和麝香鸭中研究了 Sophy β-葡聚糖（SBG）的佐剂效应，SBG 是一种由黑酵母 Aureobasidium pollulans 菌株 AF0-202 产生的β-1,3-1,6 葡聚糖，与 H5 亚型禽流感疫苗联合使用。在实验 1 中，将 40 只鸡（ISA Brown 杂交）分配到四个组，每组 10 只，分别用 Oil-H5N1(VN)、Oil-H5N1(CN)、Oil-H5N2(CN)和生理盐水（对照组）免疫。在实验 2 中，使用鸡（ISA Brown 杂交）、麝香鸭（法国杂交）和越南鸭（本地越南）进一步评估 SBG 对 Oil-H5N1(VN)越南疫苗免疫原性的影响。使用酶联免疫吸附试验（ELISA）和血凝抑制（HI）试验评估抗体反应。与未使用 β-葡聚糖的动物相比，H5 亚型疫苗在使用 SBG 的动物中引发了更高的免疫反应，HI 滴度高 1.0-1.5 对数，ELISA 血清转化率高 10-20%。值得注意的是，一些使用 SBG 处理的动物在加强免疫后诱导的 HI 滴度高于 9.0 对数。综上所述，我们的系列研究表明，SBG 是一种潜在的效应物，如增强对所测试 H5 疫苗的免疫反应。
