Liu Shaofeng, Li Wen, Chen Yan, Lin Qin, Wang Zhengmin, Li Huawei
Otology Skull base Surgery Department, Hearing Research Institute, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai, PR China.
Neuroreport. 2011 Jun 11;22(8):396-401. doi: 10.1097/WNR.0b013e328346c10f.
To investigate roles of bone morphogenetic protein (BMP) signaling on the development of mouse cochlear sensory organ, we isolated initial auditory epithelium from embryo and cultured with agonist or antagonist of BMP signaling. When BMP signaling was blocked with noggin cochlear epithelia were disordered, with hair cells in abnormal arrangement and disheveled hair bundles. The number of myosin VIIA and SRY (sex-determining region Y)-box 2 (SOX2)-positive cells also decreased dramatically. Conversely, an increase of myosin VIIA and SRY (sex-determining region Y)-box 2-positive cells was observed when auditory epithelia were treated with exogenous BMP4. Cell proliferation and cell death were not changed significantly by BMP signaling. Collectively, our results indicated that BMP signaling is essential to cochlear sensory formation and hair cell differentiation.
为了研究骨形态发生蛋白（BMP）信号通路在小鼠耳蜗感觉器官发育中的作用，我们从胚胎中分离出初始听觉上皮，并使用BMP信号通路的激动剂或拮抗剂进行培养。当用诺金蛋白阻断BMP信号通路时，耳蜗上皮紊乱，毛细胞排列异常，毛束杂乱。肌球蛋白VIIA和SRY（性别决定区Y）-盒2（SOX2）阳性细胞的数量也显著减少。相反，当用外源性BMP4处理听觉上皮时，观察到肌球蛋白VIIA和SRY（性别决定区Y）-盒2阳性细胞增加。BMP信号通路对细胞增殖和细胞死亡没有显著影响。总的来说，我们的结果表明，BMP信号通路对耳蜗感觉形成和毛细胞分化至关重要。
