UNIV OCCUPAT & ENVIRONM HLTH,SCH MED,DEPT PATHOL,YAHATANISHI KU,KITAKYUSHU,FUKUOKA 807,JAPAN. KURUME UNIV,SCH MED,DEPT SURG,KURUME,FUKUOKA 830,JAPAN. KURUME UNIV,SCH MED,DEPT PATHOL,KURUME,FUKUOKA 830,JAPAN.
Int J Oncol. 1997 Feb;10(2):269-77. doi: 10.3892/ijo.10.2.269.
We investigated the effects of epidermal growth factor (EGF) and transforming growth factor-beta (TGF-beta) on cell growth and on regulation of matrix metalloproteinases (MMPs) in four cell lines of human esophageal squamous cell carcinomas (TE8, TE9, TE10, and TE11). EGF stimulated the production of proforms of gelatinase B (MMP-9) by three cell lines that could synthesize EGF by themselves, with TE9 being the exception. Particularly, both the production of MMP-9 and DNA synthesis in TE10 were stimulated significantly by EGF. TGF-beta slightly stimulated DNA synthesis in two cell lines, TE9 and TE11, and TGF-beta secretion by TE9 was detected. The production of proforms of gelatinases A (MMP-2) and MMP-9 was gradually induced by TGF-beta in a concentration-dependent manner in all the cell lines except for TE9. Flow cytometric analysis revealed that all the lines expressed both EGF- and TGF-beta-receptors. In conclusion, our present results indicate that at least there are possibly two distinct phenotypes in human esophageal squamous cell carcinoma: one (TE10) depends on autocrine EGF production that enhances DNA synthesis and MMP-9 production; and the other (TE9) on autocrine TGF-beta that stimulates DNA synthesis but not in relation to gelatinase production.
我们研究了表皮生长因子（EGF）和转化生长因子-β（TGF-β）对 4 个人类食管鳞癌细胞系（TE8、TE9、TE10 和 TE11）细胞生长和基质金属蛋白酶（MMPs）调节的影响。EGF 刺激了能够自身合成 EGF 的 3 个细胞系产生明胶酶 B（MMP-9）的前体，TE9 除外。特别是，EGF 显著刺激了 TE10 中 MMP-9 的产生和 DNA 合成。TGF-β 轻微刺激了 2 个细胞系 TE9 和 TE11 的 DNA 合成，并检测到了 TE9 分泌的 TGF-β。除了 TE9 之外，所有细胞系中的 TGF-β 以浓度依赖的方式逐渐诱导明胶酶 A（MMP-2）和 MMP-9 前体的产生。流式细胞术分析表明，所有细胞系均表达 EGF 和 TGF-β 受体。总之，我们的研究结果表明，人类食管鳞癌至少存在两种不同的表型：一种（TE10）依赖于自分泌 EGF 产生，增强 DNA 合成和 MMP-9 产生；另一种（TE9）依赖于自分泌 TGF-β，刺激 DNA 合成，但与明胶酶产生无关。
