Department of Microbiology, and Molecular Science and Technology, Ajou University School of Medicine, Suwon, Korea.
Parasite Immunol. 2011 Jul;33(7):382-9. doi: 10.1111/j.1365-3024.2011.01296.x.
Free-living Naegleria fowleri causes primary amoebic meningoencephalitis (PAM) in humans and animals. To examine the effect of immunization with Nfa1 protein on experimental murine PAM because of N. fowleri, BALB/c mice were intra-peritoneally or intra-nasally immunized with a recombinant Nfa1 protein. We analysed Nfa1-specific antibody and cytokine induction, and the mean survival time of infected mice. Mice immunized intra-peritoneally or intra-nasally with rNfa1 protein developed specific IgG, IgA and IgE antibodies; the IgG response was dominated by IgG1, followed by IgG2b, IgG2a and IgG3. High levels of the Th1 cytokine, IFN-γ, and the regulatory cytokine, IL-10, were also induced. The mean survival time of mice immunized intra-peritoneally with rNfa1 protein was prolonged compared with controls, (25.0 and 15.5 days, respectively). Similarly, the mean survival time of mice immunized intra-nasally with rNfa1 protein was 24.7 days, compared with 15.0 days for controls.
自由生活的棘阿米巴原虫引起人类和动物原发性阿米巴脑膜脑炎（PAM）。为了研究免疫接种 Nfa1 蛋白对棘阿米巴原虫引起的实验性鼠 PAM 的影响，用重组 Nfa1 蛋白对 BALB/c 小鼠进行腹膜内或鼻腔内免疫接种。我们分析了 Nfa1 特异性抗体和细胞因子的诱导以及感染小鼠的平均存活时间。用 rNfa1 蛋白腹膜内或鼻腔内免疫接种的小鼠产生了特异性 IgG、IgA 和 IgE 抗体；IgG 反应主要由 IgG1 主导，其次是 IgG2b、IgG2a 和 IgG3。高水平的 Th1 细胞因子 IFN-γ和调节性细胞因子 IL-10 也被诱导。用 rNfa1 蛋白腹膜内免疫接种的小鼠的平均存活时间比对照组延长（分别为 25.0 和 15.5 天）。同样，用 rNfa1 蛋白鼻腔内免疫接种的小鼠的平均存活时间为 24.7 天，而对照组为 15.0 天。
