Dept. of Agro-Environmental Sciences, Chemistry and Crop Protection, Faculty of Agricultural Science, Univ. of Foggia, Via Napoli 25, 71100 Foggia, Italy.
J Food Sci. 2010 Sep;75(7):M430-6. doi: 10.1111/j.1750-3841.2010.01742.x. Epub 2010 Sep 2.
The purpose of this study was to evaluate the use of Lactobacillus plantarum, isolated from table olives "Bella di Cerignola," a traditional variety of Apulian region (Southern Italy), as a starter for this kind of food. We focused on the interaction of the starter with the natural occurring microflora, the quantitative/qualitative composition of yeast population, the decrease of pH, and the content of organic acids. After a preliminary characterization, 3 strains of Lb. plantarum, selected for their probiotic and technological performances, were used as a multiple-strain starter and inoculated (approximately 2%) in olives, processed according to Spanish style, brined at 8% and 10% of NaCl and added with 0.5% of glucose. The combination of the starter and glucose assured a correct fermentation course, decreasing the pH up to a safe value (4.3 to 4.5) and controlled the growth of yeasts. The concentrations of both L- and D-lactic acids increased throughout the fermentation, while citric and malic acids (both the isomers D and L) remained at low levels (0.2 to 0.4 g/L). Concerning yeast species, Candida guilliermondii was mainly isolated at the beginning (7 to 14 d), while C. famata prevailed at the end of fermentation.
To the question "How to standardize and maintain quality of "Bella di Cerignola" olives (Southern Italy)" we can suggest the following answer: use Lb. plantarum and a low amount of glucose (0.5%). The result is a decrease of the pH below the safety break point.
本研究的目的是评估从传统的普利亚地区(意大利南部)“贝拉迪塞里尼奥拉”食用橄榄中分离出的植物乳杆菌作为这种食品的起始菌的用途。我们专注于起始菌与自然发生的微生物群落的相互作用、酵母种群的数量/质量组成、pH 值下降和有机酸含量。经过初步特征描述,选择了 3 株具有益生菌和技术性能的 Lb. plantarum 菌株作为多菌株起始菌,并接种(约 2%)在橄榄中,按照西班牙风格加工,用 8%和 10%的 NaCl 腌制,并添加 0.5%的葡萄糖。起始菌和葡萄糖的组合确保了正确的发酵过程,将 pH 值降低到安全值(4.3 至 4.5)并控制了酵母的生长。L-和 D-乳酸的浓度在整个发酵过程中均增加,而柠檬酸和苹果酸(均为 D 和 L 异构体)的浓度保持在较低水平(0.2 至 0.4 g/L)。关于酵母种类,在发酵开始时(7 至 14 天)主要分离出假丝酵母,而在发酵结束时假丝酵母占优势。
对于“如何规范和保持‘贝拉迪塞里尼奥拉’橄榄(意大利南部)的质量”这个问题,我们可以提出以下建议:使用 Lb. plantarum 和少量葡萄糖(0.5%)。结果是 pH 值下降到安全临界点以下。