College of Food Science and Engineering, Shandong Agricultural Univ, Taian 271018, China.
J Food Sci. 2011 Mar;76(2):R69-75. doi: 10.1111/j.1750-3841.2010.02020.x.
Immunoassay, based on a selective affinity of the biological antibody for its antigen, is one of the most usual analytical methods in food safety and environmental chemistry. However, it presents several drawbacks because of the nature of the antibody. Molecular imprinting technique, due to its high selectivity and stability, ease of preparation and low cost, has shown great potential in producing artificial antibodies in biomimetic immunoassays. This article focuses on the recent states, advantages, current problems and outlooks of molecularly imprinted radio, fluoro, enzyme-linked and chemiluminescent immunoassays, and biomimetic immunosensor, with special emphasis on the challenges in developing biomimetic enzyme-linked immunosorbent assays (BELISAs). The biomimetic immunoassay method will provide an important new analysis platform in food safety, although the sensitivity and specificity is relatively low.
As a new simple analysis method, the biomimetic immunoassay has attractive prospect, although some limitations were existed in real-sample assay. In this critical review, some promising solutions for overcoming its drawbacks were put forward, which may promote the more quick development and extensive application of this method in food safety.
免疫分析基于生物抗体对其抗原的选择性亲和力，是食品安全和环境化学中最常用的分析方法之一。然而，由于抗体的性质，它存在一些缺点。分子印迹技术由于其高选择性和稳定性、易于制备和低成本，在仿生免疫分析中制备人工抗体方面显示出巨大的潜力。本文重点介绍了近年来分子印迹放射性、荧光、酶联和化学发光免疫分析和仿生免疫传感器的最新进展、优点、当前问题和展望，特别强调了开发仿生酶联免疫吸附测定（BELISAs）的挑战。仿生免疫分析方法将为食品安全提供一个重要的新分析平台，尽管灵敏度和特异性相对较低。
作为一种新的简单分析方法，仿生免疫分析具有吸引力，尽管在实际样品分析中存在一些局限性。在这篇评论中，提出了一些有希望的解决方案来克服其缺点，这可能会促进该方法在食品安全领域的更快发展和更广泛的应用。
