de Miranda J R, Thomas M A, Thurman D A, Tomsett A B
Department of Genetics and Microbiology, University of Liverpool, England.
FEBS Lett. 1990 Jan 29;260(2):277-80. doi: 10.1016/0014-5793(90)80122-y.
In response to excess metal, higher plants produce metal-binding peptides [( gamma EC]nG) whose biosynthesis is believed to be mediated by enzymes involved in glutathione (gamma ECG) metabolism. In contrast, animals synthesize metallothioneins, gene-encoded low molecular weight cysteine-rich metal-binding proteins. In an investigation of copper-regulated genes in the copper-tolerant flowering plant Mimulus guttatus, we have isolated a series of cDNA clones identifying two genes which encode a protein with class I metallothionein domains. This represents the first description of a metallothionein gene in a flowering plant.
作为对过量金属的响应，高等植物会产生金属结合肽[（γEC）nG]，其生物合成被认为是由参与谷胱甘肽（γECG）代谢的酶介导的。相比之下，动物合成金属硫蛋白，这是一类由基因编码的富含半胱氨酸的低分子量金属结合蛋白。在对耐铜开花植物黄花沟酸浆中铜调节基因的研究中，我们分离出了一系列cDNA克隆，鉴定出两个编码具有I类金属硫蛋白结构域的蛋白质的基因。这是开花植物中金属硫蛋白基因的首次描述。
