对掺入大鼠晶状体DNA中的3H-胸腺嘧啶进行液体闪烁计数。

Liquid scintillation counting of 3H-thymidine incorporated into rat lens DNA.

作者信息

Söderberg P G, Lindström B

机构信息

Department of Medical Biophysics, Karolinska Institute, Stockholm, Sweden.

出版信息

Int J Rad Appl Instrum A. 1990;41(1):1-4. doi: 10.1016/0883-2889(90)90122-w.

DOI:10.1016/0883-2889(90)90122-w

PMID:2153646

Abstract

DNA synthesis in the lens has previously been localized by autoradiography following incorporation of 3H-thymidine. For the quantification of DNA synthesis in the lens, pooling of lenses and extraction of the DNA for liquid scintillation counting, has formerly been adapted. In the present investigation a method has been developed for the extraction of the unincorporated tracer from whole lenses after short time incubation in a medium containing 3H-thymidine. The 3H-thymidine incorporated into individual lenses was then detected by liquid scintillation counting after dissolution of the lenses. The sources of the variation in the method are evaluated.

摘要

晶状体中的DNA合成此前已通过掺入³H-胸腺嘧啶核苷后的放射自显影进行定位。为了定量晶状体中的DNA合成，以前采用将晶状体合并并提取DNA用于液体闪烁计数的方法。在本研究中，开发了一种方法，用于在含有³H-胸腺嘧啶核苷的培养基中短时间孵育后从整个晶状体中提取未掺入的示踪剂。然后在晶状体溶解后通过液体闪烁计数检测掺入单个晶状体中的³H-胸腺嘧啶核苷。对该方法中变异的来源进行了评估。