Bermudes Fernando Antônio Martins, Dettoni Juliano Bertollo, Pereira Fausto Edmundo Lima
Department of Surgery, Health Sciences Center, UFES, Brazil.
Acta Cir Bras. 2011 Jun;26(3):181-5. doi: 10.1590/s0102-86502011000300005.
To investigate the effect of 72 hours food suppression on the evolution of fecal peritonitis in mice evaluating the mortality and measuring the number and size of abscesses formed into the peritoneal cavity.
Mice receiving commercial diet and water ad libitum (control group, N=35) and mice fasted during 72 h (N=35), receiving only water ad libitum, were inoculated by i.p. route, with 4uL/g body weight of a fecal suspension diluted 1:6 or 1:9 in 0.15M NaCl solution (1:6 dilution, 22 controls and 18 fasted; 1:9 dilution, 13 controls and 17 fasted). Animals were followed up until two weeks after fecal inoculation, when the survivors were euthanized for evaluation of the number and size of intra-peritoneal abscesses. Mortality was evaluated by Kaplan Meyer curves.
Mortality was significantly higher in fasted groups than in controls. However the number and size of abscesses were significantly less in fasted groups than in controls.
Seventy two hours food suppression increased the susceptibility to endotoxic shock (high mortality after peritonitis induction) and the resistance to infection with fecal microorganisms (less number and size of intra-peritoneal abscesses).
通过评估死亡率并测量腹腔内形成的脓肿数量和大小，研究72小时食物限制对小鼠粪便性腹膜炎演变的影响。
自由摄取商业饮食和水的小鼠（对照组，N = 35）以及禁食72小时（N = 35）、仅自由摄取水的小鼠，通过腹腔注射途径，按4μL/g体重接种在0.15M NaCl溶液中稀释1:6或1:9的粪便悬液（1:6稀释，22只对照和18只禁食；1:9稀释，13只对照和17只禁食）。对动物进行随访直至粪便接种后两周，此时对存活者实施安乐死以评估腹腔内脓肿的数量和大小。通过Kaplan Meyer曲线评估死亡率。
禁食组的死亡率显著高于对照组。然而，禁食组脓肿的数量和大小显著少于对照组。
72小时食物限制增加了对内毒素休克的易感性（腹膜炎诱导后死亡率高）以及对粪便微生物感染的抵抗力（腹腔内脓肿数量和大小较少）。
