Brath Endre, Miko Iren, Nemeth Norbert, Kovacs Judit, Peto Katalin, Furka Istvan
Department of Operative Techniques and Surgical Research, Institute of Surgery, Medical and Health Science Center, University of Debrecen, Hungary.
Acta Cir Bras. 2011 Jun;26(3):186-93. doi: 10.1590/s0102-86502011000300006.
To investigate the duration of apoptosis caused by ischemia-reperfusion in the intestine in a new double jejunum-segment model, and to analyze the protective effects of allopurinol or ischemic preconditioning (IPC).
In Experiment I for harvesting the double jejunum-segment model after laparotomy a 30-cm-long jejunum part was selected on mongrel dogs (n=24). End-to-end anastomoses were performed at both ends and in the middle of the jejunum part, creating two equal segments. In one segment ischemia was induced by occluding the supplying vessels, the other segment served as control. Tissue samples for detecting apoptosis were taken at 30th minutes, 1st, 2nd, 4th, 6th, 8th, 12th and 24th hours of reperfusion. In Experiment II using the same model the 4-hour reperfusion time period, allopurinol (50 mg/kg) pre-treated and IPC (3 cycles of 5x1) groups (n=5 per each) were also investigated.
In Experiment I the greatest apoptotic activity was detected at the 4th and 6th hour of reperfusion (14.2 ± 1.31 and 16.3 ± 1.05 per visual field at 40x magnification). In Experiment II Using the 4-hour reperfusion time period allopurinol pre-treatment increased the apoptotic activity (10.72 ± 0.47 per 50 intestinal villi) approximately two-fold than the IPC (6.72 ± 0.46 per 50 intestinal villi) did (p<0.05).
Apoptotic activity has a characteristic time curve, reaching the highest values between the 4th and 6th hours after 30-minute intestinal ischemia. Ischemic preconditioning seemed to be protective against the morphological changes caused by intestinal ischemia-reperfusion.
在一种新的双空肠段模型中研究肠缺血再灌注引起的细胞凋亡持续时间，并分析别嘌呤醇或缺血预处理（IPC）的保护作用。
在实验I中，剖腹术后选取杂种犬（n = 24）一段30厘米长的空肠部分制作双空肠段模型。在空肠部分的两端及中间进行端端吻合，形成两个等长的肠段。一段通过阻断供血血管诱导缺血，另一段作为对照。在再灌注的第30分钟、1小时、2小时、4小时、6小时、8小时、12小时和24小时采集用于检测细胞凋亡的组织样本。在实验II中，使用相同模型，还研究了4小时再灌注时间段内别嘌呤醇（50mg/kg）预处理组和缺血预处理（3个5×1周期）组（每组n = 5）。
在实验I中，再灌注第4小时和第6小时检测到最大的细胞凋亡活性（40倍放大视野下分别为14.2±1.31和16.3±1.05）。在实验II中，使用4小时再灌注时间段，别嘌呤醇预处理使细胞凋亡活性（每50个肠绒毛10.72±0.47）比缺血预处理（每50个肠绒毛6.72±0.46）增加了约两倍（p<0.05）。
细胞凋亡活性具有特征性的时间曲线，在肠缺血30分钟后第4小时至第6小时达到最高值。缺血预处理似乎对肠缺血再灌注引起的形态学变化具有保护作用。
