Vilela Márcia Carvalho, Campos Roberta Dayrell de Lima, Mansur Daniel Santos, Rodrigues David Henrique, Lacerda-Queiroz Norinne, Lima Graciela Kunrath, Rachid Milene Alvarenga, Kroon Erna Geessien, Campos Marco Antônio, Teixeira Antônio Lúcio
Laboratório de Imunofarmacologia, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, MG, Brazil.
Arq Neuropsiquiatr. 2011 Apr;69(2A):237-41. doi: 10.1590/s0004-282x2011000200019.
Herpes simplex virus-1 (HSV-1) is a pathogen that may cause severe encephalitis in humans. In this study, we aimed to investigate the role of interleukin-4 (IL-4) in a model of HSV-1 brain infection. IL-4 knockout (IL-4-/-) and wild type (WT) C57BL/6 mice were inoculated with 10(4) plaque-forming units of HSV-1 by the intracranial route. Histopathologic analysis revealed a distinct profile of infiltrating cells at 3 days post-infection (dpi). Infected WT mice presented mononuclear inflammatory cells while IL-4-/- mice developed meningoencephalitis with predominance of neutrophils. IL-4-/- mice had diminished leukocyte adhesion at 3 dpi when compared to infected WT animals in intravital microscopy study. Conversely no differences were found in cerebral levels of CXCL1, CXCL9, CCL3, CCL5 and TNF-α between WT and IL-4-/- infected mice. IL-4 may play a role in the recruitment of cells into central nervous system in this acute model of severe encephalitis caused by HSV-1.
单纯疱疹病毒1型（HSV-1）是一种可导致人类严重脑炎的病原体。在本研究中，我们旨在研究白细胞介素-4（IL-4）在HSV-1脑感染模型中的作用。通过颅内途径给IL-4基因敲除（IL-4-/-）和野生型（WT）C57BL/6小鼠接种10⁴个HSV-1空斑形成单位。组织病理学分析显示感染后3天（dpi）浸润细胞的特征明显不同。感染的WT小鼠出现单核炎性细胞，而IL-4-/-小鼠则发展为以中性粒细胞为主的脑膜脑炎。在活体显微镜研究中，与感染的WT动物相比，IL-4-/-小鼠在3 dpi时白细胞黏附减少。相反，WT和IL-4-/-感染小鼠的大脑中CXCL1、CXCL9、CCL3、CCL5和TNF-α水平没有差异。在这种由HSV-1引起的严重脑炎急性模型中，IL-4可能在细胞向中枢神经系统的募集过程中发挥作用。
