Castro-Raucci Larissa Moreira Spinola de, Oliveira Ivone Regina de, Teixeira Lucas Novaes, Rosa Adalberto Luiz, Oliveira Paulo Tambasco de, Jacobovitz Marcos
Ribeirão Preto Dental School, University of São Paulo, SP, Brazil.
Braz Dent J. 2011;22(2):99-104. doi: 10.1590/s0103-64402011000200002.
The present study evaluated the progression of osteogenic cell cultures exposed to a novel calcium aluminate cement (CAC+) in comparison with the gold standard mineral trioxide aggregate (MTA). Cells were enzimatically isolated from newborn rat calvarial bone, plated on glass coverslips containing either CAC+ or a control MTA samples in the center, and grown under standard osteogenic conditions. Over the 10-day culture period, roundening of sample edges was clearly noticed only for MTA group. Although both cements supported osteogenic cell adhesion, spreading, and proliferation, CAC+-exposed cultures showed significantly higher values in terms of total cell number at days 3 and 7, and total protein content and alkaline phosphatase activity at day 10. The present in vitro results indicate that the exposure to CAC+ supports a higher differentiation of osteogenic cells compared with the ones exposed to MTA. Further experimental studies should consider CAC+ as a potential alternative to MTA when the repair of mineralized tissues is one of the desired outcomes in endodontic therapy.
本研究评估了与金标准三氧化矿物凝聚体（MTA）相比，暴露于新型铝酸钙水泥（CAC+）的成骨细胞培养物的进展情况。细胞从新生大鼠颅骨中酶解分离，接种于中央含有CAC+或对照MTA样本的玻璃盖玻片上，并在标准成骨条件下培养。在10天的培养期内，仅MTA组明显观察到样本边缘变圆。虽然两种水泥都支持成骨细胞的黏附、铺展和增殖，但暴露于CAC+的培养物在第3天和第7天的总细胞数以及第10天的总蛋白含量和碱性磷酸酶活性方面显示出显著更高的值。目前的体外结果表明，与暴露于MTA的细胞相比，暴露于CAC+的细胞能支持更高程度的成骨细胞分化。当矿化组织修复是牙髓治疗期望达到的结果之一时，进一步的实验研究应考虑将CAC+作为MTA的潜在替代品。
