Department of Endodontics, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-8310, Japan.
Dent Mater J. 2012 Feb 3;31(1):92-7. doi: 10.4012/dmj.2011-151. Epub 2012 Jan 21.
The aim of this study was to investigate mineralizing ability of a premixed calcium phosphate cement (premixed-CPC) compared to mineral trioxide aggregate (MTA) and zinc oxide eugenol cement (SuperEBA) in ROS17/2.8 cells. The measurements of cell proliferation, alkaline phosphatase (ALPase) activity and mineralized nodule formation in the presence or absence (control) of the test materials were performed using a cell culture insert method with the test materials placed on a porous membrane of culture plate insert. Mineralized nodules were detected by staining with alizarin red, and the calcium content of the mineralized nodules was determined quantitatively using a calcium assay kit. Premixed-CPC and MTA indicated significantly higher cell proliferation, ALPase activity, mineralized nodule formation, and calcium content in nodules than those of SuperEBA (p<0.05). The present results suggest that premixed-CPC has the same mineralizing ability as MTA.
本研究旨在比较预混磷酸钙水泥（预混-CPC）与矿化三氧化物凝聚体（MTA）和氧化锌丁香酚水门汀（SuperEBA）在 ROS17/2.8 细胞中的矿化能力。采用细胞培养插入法，将测试材料置于培养板插入的多孔膜上，在存在或不存在（对照）测试材料的情况下，测量细胞增殖、碱性磷酸酶（ALPase）活性和矿化结节形成。通过茜素红染色检测矿化结节，使用钙测定试剂盒定量测定矿化结节中的钙含量。预混-CPC 和 MTA 表明细胞增殖、ALPase 活性、矿化结节形成和结节中的钙含量均显著高于 SuperEBA（p<0.05）。本研究结果表明，预混-CPC 具有与 MTA 相同的矿化能力。
