肌萎缩侧索硬化症中的神经元基因表达

Neuronal gene expression in amyotrophic lateral sclerosis.

作者信息

Clark A W, Tran P M, Parhad I M, Krekoski C A, Julien J P

机构信息

Department of Pathology, University of Calgary, Alta, Canada.

出版信息

Brain Res Mol Brain Res. 1990 Jan;7(1):75-83. doi: 10.1016/0169-328x(90)90076-p.

DOI:10.1016/0169-328x(90)90076-p

PMID:2153897

Abstract

To characterize neuronal gene expression in amyotrophic lateral sclerosis (ALS), we quantitated one glial and three neuronal mRNAs in spinal cords of 7 subjects with ALS and 11 controls. The ALS cases showed no loss of mRNA for the neurofilament light subunit when assessed with in situ hybridization. Northern analysis, and RNase protection assay; and no loss of mRNA for amyloid precursor protein or a growth-associated protein (GAP-43/B-50) on Northern analysis. ALS cords also showed no significant change in glial mRNA. Our findings indicate that expression of these neuronal mRNAs is well maintained in ALS-afflicted spinal cord. They do not support the hypothesis of a generalized impairment of neuronal gene transcription in the pathogenesis of this disorder.

摘要

为了描述肌萎缩侧索硬化症（ALS）中神经元基因的表达情况，我们对7例ALS患者和11例对照者脊髓中的一种胶质细胞mRNA和三种神经元mRNA进行了定量分析。通过原位杂交、Northern印迹分析和核糖核酸酶保护分析评估，ALS患者的神经丝轻链亚基mRNA没有缺失；Northern印迹分析显示淀粉样前体蛋白或生长相关蛋白（GAP-43/B-50）的mRNA也没有缺失。ALS患者的脊髓中胶质细胞mRNA也没有显著变化。我们的研究结果表明，这些神经元mRNA的表达在ALS累及的脊髓中得到了很好的维持。它们不支持在这种疾病发病机制中神经元基因转录普遍受损的假说。