Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
J Ethnopharmacol. 2011 Jun 14;136(1):55-66. doi: 10.1016/j.jep.2011.03.061. Epub 2011 Apr 20.
Gimjeng and Chakapat lychee (Litchi chinensis Sonn.) were evaluated for hepatoprotective activity on CCl(4)-induced hepatotoxicity in rats.
Fruit pulp extracts of the lychees were examined for vitamin C, phenolic contents, anti-lipid peroxidation activity and hepatoprotective effect. Male Wistar albino rats were intraperitoneally injected (ip) with CCl(4) (2 ml/kg), then were orally administered (po) with silymarin (100mg/kg), and Gimjeng or Chakapat extracts (100 and 500 mg/kg). After ten days, the rats were sacrificed and their livers were examined histopathologically and immunohistochemically. Their serum glutamate-pyruvate transaminase, glutamate-oxalate transaminase, and alkaline phosphatase activities were analyzed. Apoptotic activity of the livers was assessed quantitatively.
The Gimjeng and Chakapat extracts showed the contents of vitamin C (1.2±0.6 and 4.3±0.1mg/100g) and phenolics like trans-cinnamic acid and pelargonidin-3-O-glucoside (9.80±0.21 and 19.56±0.4 mg GAE/g extract, respectively), and trolox equivalent antioxidant capacity (TEAC) values (11.64 and 9.09 g/mg trolox), respectively. The Gimjeng as compared to the Chakapat demonstrated a better antioxidant activity as revealed by anti-lipid peroxidation activity with the TEAC values. Administration of CCl(4) in rats elevated the serum GPT, GOT, and ALP level whereas silymarin, Gimjeng and Chakapat extracts prevented these increases significantly. Significant decrease of apoptotic cells together with restoration of morphological changes confirmed the hepatoprotective effect in the CCl(4)-induced rats pretreated with the extracts.
Antioxidant properties of the Gimjeng and Chakapat lychees as evidenced by the vitamin C and phenolic compounds, anti-lipid peroxidation and anti-apoptosis could explain the hepatoprotective effects in CCl(4)-induced hepatotoxicity.
评估 Gimjeng 和 Chakapat 荔枝（Litchi chinensis Sonn.）对 CCl 4 诱导的大鼠肝毒性的肝保护活性。
对荔枝的果肉提取物进行了维生素 C、酚类含量、抗脂质过氧化活性和肝保护作用的检测。雄性 Wistar 白化大鼠腹腔注射（ip）CCl 4（2ml/kg），然后口服给予水飞蓟素（100mg/kg）和 Gimjeng 或 Chakapat 提取物（100 和 500mg/kg）。十天后，处死大鼠，对其肝脏进行组织病理学和免疫组织化学检查。分析血清谷氨酸-丙酮酸转氨酶、谷氨酸-草酰乙酸转氨酶和碱性磷酸酶活性。定量评估肝脏的凋亡活性。
Gimjeng 和 Chakapat 提取物分别含有维生素 C（1.2±0.6 和 4.3±0.1mg/100g）和肉桂酸和矢车菊素-3-O-葡萄糖苷等酚类物质（9.80±0.21 和 19.56±0.4mg GAE/g 提取物），以及 Trolox 当量抗氧化能力（TEAC）值（11.64 和 9.09g/mg Trolox）。与 Chakapat 相比，Gimjeng 表现出更好的抗氧化活性，这体现在 TEAC 值的抗脂质过氧化活性上。CCl 4 处理大鼠可使血清 GPT、GOT 和 ALP 水平升高，而水飞蓟素、Gimjeng 和 Chakapat 提取物可显著阻止这些升高。提取物预处理 CCl 4 诱导的大鼠，凋亡细胞数量减少，形态学变化得到恢复，证实了其具有肝保护作用。
Gimjeng 和 Chakapat 荔枝的抗氧化特性，如维生素 C 和酚类化合物、抗脂质过氧化和抗细胞凋亡，可解释其在 CCl 4 诱导的肝毒性中的肝保护作用。
