Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC-Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD 21702, United States.
J Proteomics. 2011 Nov 18;74(12):2650-9. doi: 10.1016/j.jprot.2011.04.011. Epub 2011 Apr 21.
The search for protein biomarkers has been a highly pursued topic in the proteomics community in the last decade. This relentless search is due to the constant need for validated biomarkers that could facilitate disease risk stratification, disease diagnosis, prognosis, monitoring as well as drug development, which ultimately would improve our quality of life. The recent development of proteomic technologies including the advancement of mass spectrometers with high sensitivity and speed has greatly advanced the discovery of potential biomarkers. One of the bottlenecks lies in the development of well-established verification assays to screen the biomarker candidates identified in the discovery stage. Recently, absolute quantitation using multiple-reaction monitoring mass spectrometry (MRM-MS) in combination with isotope-labeled internal standards has been extensively investigated as a tool for high-throughput protein biomarker verification. In this review, we describe and discuss recent developments and applications of MRM-MS methods for biomarker verification.
在过去的十年中，蛋白质生物标志物的研究一直是蛋白质组学领域的热门话题。这种不懈的研究源于对经过验证的生物标志物的持续需求，这些生物标志物可以促进疾病风险分层、疾病诊断、预后、监测以及药物开发，从而最终提高我们的生活质量。近年来，蛋白质组学技术的发展，包括灵敏度和速度更高的质谱仪的进步，极大地推动了潜在生物标志物的发现。其中一个瓶颈在于开发经过良好验证的验证检测方法，以筛选在发现阶段中确定的生物标志物候选物。最近，使用多重反应监测质谱（MRM-MS）结合同位素标记内标进行绝对定量已被广泛研究作为高通量蛋白质生物标志物验证的工具。在本文中，我们描述并讨论了 MRM-MS 方法在生物标志物验证中的最新进展和应用。
