核苷酸焦磷酸酶在恩氟烷诱导小鼠肝脏中尿苷二磷酸葡萄糖醛酸浓度降低中的作用。

Role of nucleotide pyrophosphatase in enflurane-induced reduction of UDP-glucuronic acid concentration in mouse liver.

作者信息

Watkins J B, Pierce M A

机构信息

Medical Science Program, Indiana University School of Medicine, Bloomington 47405.

出版信息

Toxicol Appl Pharmacol. 1990 Feb;102(2):378-83. doi: 10.1016/0041-008x(90)90034-r.

DOI:10.1016/0041-008x(90)90034-r

PMID:2154067

Abstract

Because hepatic UDP-glucuronic acid levels decrease upon exposure to volatile anesthetics, the present study was designed to determine the mechanism by which enflurane decreases UDP-glucuronic acid in mice by measuring the concentrations of intermediates and the activities of enzymes in the UDP-glucuronic acid pathway. UDP-glucuronic acid concentrations were decreased by 40% in both male and female mice after 10 min of enflurane-induced narcosis. Concentration of UDP-glucose and the activities of diethylstilbestrol UDP-glucuronosyltransferase and UDP-glucose dehydrogenase were not affected by enflurane treatment. In contrast, nucleotide pyrophophatase activity was increased approximately 50% in both sexes. Thus, the decrease in hepatic UDP-glucuronic acid upon exposure of mice to enflurane is probably due to increased degradation by nucleotide pyrophosphatase.

摘要

由于暴露于挥发性麻醉剂后肝脏UDP-葡萄糖醛酸水平会降低，本研究旨在通过测量UDP-葡萄糖醛酸途径中中间体的浓度和酶的活性，来确定恩氟烷降低小鼠UDP-葡萄糖醛酸的机制。恩氟烷诱导麻醉10分钟后，雄性和雌性小鼠的UDP-葡萄糖醛酸浓度均降低了40%。UDP-葡萄糖的浓度以及己烯雌酚UDP-葡萄糖醛酸基转移酶和UDP-葡萄糖脱氢酶的活性不受恩氟烷处理的影响。相比之下，核苷酸焦磷酸酶活性在两性中均增加了约50%。因此，小鼠暴露于恩氟烷后肝脏UDP-葡萄糖醛酸的降低可能是由于核苷酸焦磷酸酶降解增加所致。