Chen Jeen-Kuan, Shen Chia-Rui, Yeh Chao-Hsien, Fang Bing-Shiun, Huang Tung-Li, Liu Chao-Lin
Environment and Biotechnology Department, Refining and Manufacturing Research Institute, CPC Corporation, Taiwan, Chia-yi, Taiwan; E-Mail:
Int J Mol Sci. 2011 Feb 17;12(2):1187-95. doi: 10.3390/ijms12021187.
A novel chitin-degrading aerobe, Chitinibacter tainanensis, was isolated from a soil sample from southern Taiwan, and was proved to produce N-acetyl glucosamine (NAG). Chitin degrading factors (CDFs) were proposed to be the critical factors to degrade chitin in this work. When C. tainanensis was incubated with chitin, CDFs were induced and chitin was converted to NAG. CDFs were found to be located on the surface of C. tainanensis. N-Acetylglucosaminidase (NAGase) and endochitinase activities were found in the debris, and the activity of NAGase was much higher than that of endochitinase. The optimum pH of the enzymatic activity was about 7.0, while that of NAG production by the debris was 5.3. These results suggested that some factors in the debris, in addition to NAGase and endochitinase, were crucial for chitin degradation.
从台湾南部的土壤样本中分离出一种新型的几丁质降解需氧菌——台南几丁质杆菌，已证实其可产生N-乙酰葡糖胺（NAG）。在本研究中，几丁质降解因子（CDFs）被认为是降解几丁质的关键因素。当台南几丁质杆菌与几丁质一起培养时，会诱导产生CDFs，几丁质也会转化为NAG。发现CDFs位于台南几丁质杆菌的表面。在碎片中发现了N-乙酰葡糖胺酶（NAGase）和内切几丁质酶的活性，且NAGase的活性远高于内切几丁质酶。酶活性的最适pH约为7.0，而碎片产生NAG的最适pH为5.3。这些结果表明，除了NAGase和内切几丁质酶外，碎片中的一些因素对几丁质降解也至关重要。
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