来自印度奥里萨邦塔塔帕尼温泉的几株产脂肪酶嗜热细菌的生化特性及16S rRNA测序

Biochemical Characterization and 16S rRNA Sequencing of Few Lipase-Producing Thermophilic Bacteria from Taptapani Hot Water Spring, Orissa, India.

作者信息

Bisht Satpal S, Panda Amrita K

机构信息

Department of Biotechnology, Roland Institute of Pharmaceutical Sciences, Orissa, Berhampur 760010, India.

出版信息

Biotechnol Res Int. 2011 Apr 14;2011:452710. doi: 10.4061/2011/452710.

DOI:10.4061/2011/452710

PMID:21541249

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3085305/

Abstract

Three lipase-producing thermophilic bacteria (AK-P1, AK-P2, and AK-P3) were isolated from the Taptapani hot water spring in Orissa, India. The crude extra cellular lipases from cell-free culture supernatant were reacted in an olive oil mixture, and their lipolytic activities were compared. Identification of the bacteria was carried out using biochemical tests, 16SrRNA sequencing and sequences submitted to NCBI GenBank. Strain AK-P3, exhibited the highest lipolytic activity of 5.5 U/mL was identified as Porphyrobacter sp. The lipolytic activities of strains AK-P1 and AK-P 2 were 4.5 U/mL and 3.5 U/mL, respectively. Strains AK-P1 and AK-P2 were identified as Acinetobacter sp. and Brevibacillus spp. The GenBank accession numbers of the 16S rRNA gene sequences determined in this study for the strains AK-P1, AK-P2, and AK-P3 are HM359120, HM359119, and HM359118, respectively.

摘要

从印度奥里萨邦的塔塔帕尼温泉中分离出三株产脂肪酶的嗜热细菌（AK-P1、AK-P2和AK-P3）。将无细胞培养上清液中的粗细胞外脂肪酶与橄榄油混合物反应，并比较它们的脂解活性。通过生化试验、16SrRNA测序以及提交至NCBI基因库的序列对细菌进行鉴定。表现出最高脂解活性5.5 U/mL的菌株AK-P3被鉴定为卟啉杆菌属。菌株AK-P1和AK-P2的脂解活性分别为4.5 U/mL和3.5 U/mL。菌株AK-P1和AK-P2分别被鉴定为不动杆菌属和短短芽孢杆菌属。本研究中测定的菌株AK-P1、AK-P2和AK-P3的16S rRNA基因序列的GenBank登录号分别为HM359120、HM359119和HM359118。