Gassmann M, Hosang M, Mous J
Central Research Units, F. Hoffmann-La Roche & Co. Ltd. Basel, Switzerland.
Biochem Biophys Res Commun. 1990 Jan 15;166(1):154-9. doi: 10.1016/0006-291x(90)91924-h.
Platelet-derived growth factor (PDGF) consists of three different isoforms, PDGF-AA, PDGF-AB and PDGF-BB, which bind to at least two types of receptors: the B-receptor, to which only PDGF-BB binds, and the A/B receptor, to which all three isoforms bind. Microinjection of synthetic mRNA in Xenopus laevis oocytes was used to obtain cell-surface expression of the human PDGF B-receptor. The production of receptor molecules of correct size (190 kd) was demonstrated by specific immunoprecipitation; the binding properties of the membrane- associated PDGF B-receptor were investigated with highly purified recombinant [125I] labeled human PDGF-BB and -AA. Unlike Swiss mouse 3T3 cells, which possess both B- and A/B-receptors and, therefore, bind both isoforms with high affinity, the mRNA-injected oocytes bound only the BB isoform. Mock-injected oocytes showed no specific binding.
血小板衍生生长因子（PDGF）由三种不同的亚型组成，即PDGF-AA、PDGF-AB和PDGF-BB，它们至少与两种类型的受体结合：B受体，只有PDGF-BB能与之结合；A/B受体，所有三种亚型都能与之结合。通过向非洲爪蟾卵母细胞显微注射合成mRNA来实现人PDGF B受体在细胞表面的表达。通过特异性免疫沉淀证明了正确大小（190kd）的受体分子的产生；用高度纯化的重组[125I]标记的人PDGF-BB和-AA研究了膜相关PDGF B受体的结合特性。与同时拥有B受体和A/B受体、因此能以高亲和力结合两种亚型的瑞士小鼠3T3细胞不同，注射mRNA的卵母细胞只结合BB亚型。模拟注射的卵母细胞未显示特异性结合。
