Baj J, Grabowska I, Wolska K I, Markiewicz Z
Acta Microbiol Pol. 1991;40(1-2):27-35.
Amino acid analysis of pure murein isolated from cells of T. neapolitanus revealed the typical constituents of most mureins form Gram-negative bacteria. i.e. glutamic acid, alanine and diaminopimelic acid, but the molecular ratio ot these was unusual, being approximately 1: 1: 1. The reduced amount of alanine was explained by the absence of monomers containing tetrapeptide side chains, as revealed by h. p. 1. c. analysis, [(3)H]glutamic acid, [(3)H]diaminopimelic acid and [(3)H]N-acetylglucosamine were incorporated into the murein and allowed to determine the degree of its crosslinkage (28%) and the occurrence of turnover.
对从那不勒斯嗜热栖热菌细胞中分离出的纯胞壁质进行氨基酸分析，结果显示大多数革兰氏阴性菌胞壁质的典型成分，即谷氨酸、丙氨酸和二氨基庚二酸，但这些成分的分子比例并不寻常，约为1:1:1。丙氨酸含量减少的原因是缺乏含有四肽侧链的单体，这一点通过高效液相色谱分析得以揭示。[（3）H]谷氨酸、[（3）H]二氨基庚二酸和[（3）H]N-乙酰葡糖胺被掺入胞壁质中，用于确定其交联程度（28%）和周转情况。
