Department of Periodontology, School of Dentistry and Dental Research Institute, Seoul National University, Seoul, Korea.
J Periodontol. 2012 Jan;83(1):101-10. doi: 10.1902/jop.2011.110040. Epub 2011 May 4.
In previous studies, oligopeptide corresponding to the cell-binding domains of bone morphogenetic proteins that bind to bone morphogenetic protein receptor enhanced the bone regenerative capacity of bovine bone minerals (BBM). The aim of this study is to evaluate the ability of BBM coated with oligopeptide to promote periodontal regeneration in a 1-wall intrabony defect model in dogs.
The second and third mandibular premolars and first molars of six adult beagles were extracted bilaterally, and the extraction sites were allowed to heal for 10 weeks. The 1-wall intrabony defects were prepared bilaterally on the mesial and distal side of the fourth mandibular premolars. Twenty-four intrabony defects were assigned to four treatment groups: 1) open flap debridement; 2) guided tissue regeneration (GTR); 3) GTR with a collagen membrane and BBM; and 4) GTR with a collagen membrane and BBM coated with the oligopeptide (Pep-BBM). The animals were sacrificed 10 weeks after surgery. For the histometric analysis, defect height, junctional epithelium migration, new cementum, new bone height, and new bone area were measured. New bone volume was measured using microcomputed tomography.
Wound healing was generally uneventful. For junctional epithelium migration, the BBM and Pep-BBM groups exhibited mean (± SE) values of 0.53 ± 0.41 and 0.48 ± 0.30 mm, and for new cementum height, 1.71 ± 0.46 and 2.50 ± 2.00 mm, respectively. For junctional epithelium migration and cementum regeneration, there were no significant differences between the two groups. The mean (± SE) values of new bone height and new bone volume in the Pep-BBM group (3.88 ± 0.31 mm and 32.35% ± 9.60%) were significantly greater than the mean values for the BBM group (2.60 ± 0.41 mm and 20.56% ± 1.89%). For bone regeneration, the Pep-BBM group showed superior results compared to the BBM group with statistically significant differences.
Through various parameters to evaluate periodontal regeneration, this oligopeptide coating influenced only the ability of BBM to promote bone regeneration in 1-wall intrabony defects in beagles. Junctional epithelium migration and cementum regeneration were not affected by this oligopeptide coating, and further investigations with special focus on regeneration of the periodontal ligament are necessary.
在之前的研究中，与骨形态发生蛋白受体结合的骨形态发生蛋白细胞结合域的寡肽增强了牛骨矿物质（BBM）的骨再生能力。本研究旨在评估在犬 1 壁骨内缺损模型中，用寡肽涂覆 BBM 以促进牙周再生的能力。
将 6 只成年比格犬的双侧第二和第三前磨牙和第一磨牙拔出，拔牙部位愈合 10 周。在第四前磨牙的近中和远中侧双侧制备 1 壁骨内缺损。24 个骨内缺损分为 4 个治疗组：1）开放式翻瓣清创术；2）引导组织再生（GTR）；3）用胶原膜和 BBM 的 GTR；4）用胶原膜和涂覆寡肽的 BBM（Pep-BBM）的 GTR。手术后 10 周处死动物。用于组织学分析，测量缺损高度、结合上皮迁移、新牙骨质、新骨高度和新骨面积。使用微计算机断层扫描测量新骨体积。
伤口愈合总体顺利。对于结合上皮迁移，BBM 和 Pep-BBM 组的平均值（± SE）分别为 0.53 ± 0.41 和 0.48 ± 0.30mm，对于新牙骨质高度，分别为 1.71 ± 0.46 和 2.50 ± 2.00mm。对于结合上皮迁移和牙骨质再生，两组之间无显著差异。Pep-BBM 组新骨高度和新骨体积的平均值（± SE）分别为 3.88 ± 0.31mm 和 32.35%±9.60%，明显大于 BBM 组的平均值（2.60 ± 0.41mm 和 20.56%±1.89%）。对于骨再生，Pep-BBM 组的结果优于 BBM 组，差异具有统计学意义。
通过各种参数评估牙周再生，这种寡肽涂层仅影响 BBM 在比格犬 1 壁骨内缺损中促进骨再生的能力。结合上皮迁移和牙骨质再生不受这种寡肽涂层的影响，需要进一步进行特别关注牙周韧带再生的研究。
