人脑血管内皮细胞对单核细胞增生李斯特菌感染的早期基因反应。
Early gene response of human brain microvascular endothelial cells to Listeria monocytogenes infection.
机构信息
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA.
出版信息
Can J Microbiol. 2011 May;57(5):441-6. doi: 10.1139/w11-018. Epub 2011 May 5.
Abstract
The gene expression of human brain microvascular endothelial cells (HBMEC) in response to 4 h of infection by Listeria monocytogenes was analyzed. Four hours after infection, the expression of 456 genes of HBMEC had changed (p < 0.05). We noted that many active genes were involved in the formyl-methionyl-leucyl-phenylalanine pathway in infected HBMEC. In the upregulated genes, mRNA levels of interleukin-8 and interleukin-15 in infected cells increased according to microarray and real-time reverse transcription - PCR analyses. Since both cytokines are regarded as potent chemotactic factors, the results suggest that HBMEC are capable of recruiting cells of innate and adaptive immune responses during early L. monocytogenes infection.
摘要
分析了人类脑微血管内皮细胞（HBMEC）在李斯特菌属感染 4 小时后的基因表达情况。感染 4 小时后，HBMEC 的 456 个基因的表达发生了变化（p<0.05）。我们注意到，感染的 HBMEC 中许多活跃的基因参与了甲酰甲硫氨酸亮氨酸苯丙氨酸途径。在上调的基因中，根据微阵列和实时逆转录-聚合酶链反应分析，感染细胞中白细胞介素-8 和白细胞介素-15 的 mRNA 水平增加。由于这两种细胞因子都被认为是有效的趋化因子，因此结果表明，在李斯特菌属早期感染期间，HBMEC 能够招募先天和适应性免疫反应的细胞。
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