Department of Gynecology and Obstetrics, Randers County Hospital, Denmark.
Acta Obstet Gynecol Scand. 2011 Aug;90(8):897-902. doi: 10.1111/j.1600-0412.2011.01164.x. Epub 2011 Jun 14.
Pre-eclampsia is a serious pregnancy complication causing both fetal and maternal distress. Proteinuria is a diagnostic criterion frequently determined by albuminuria. We determined the protein excretion pattern of additional proteins, immunoglobulin G, transferrin, α1-microglobulin and β2-microglobulin, in urine samples collected prospectively during pre-eclamptic and healthy pregnancies.
A prospective cohort study of 1,631 consecutive pregnant women.
A Danish regional hospital.
Thirty-two women with pre-eclampsia and 185 healthy control women were identified from the cohort. Urine samples were obtained from the 18th week until delivery and divided into six gestational intervals.
Protein analyses of urine immunoglobulin G, transferrin, α1-microglobulin and β2-microglobulin were done with a sandwich ELISA method.
Urine levels of specific proteins during pre-eclamptic and healthy pregnancies.
Immunoglobulin G and transferrin were significantly increased in pre-eclampsia after the 30th week of pregnancy. α(1)-Microglobulin and β(2)-microglobulin were differently excreted and found to be higher after the 36(th) week of pregnancy in pre-eclampsia, but only α1-microglobulin increased significantly.
Immunoglobulin G, transferrin, α1- and β2-microglobulin excretion patterns indicate initial glomerular damage followed by altered tubular handling of proteins.
子痫前期是一种严重的妊娠并发症，可导致胎儿和母亲都感到不适。蛋白尿是一种常用的诊断标准，通常通过白蛋白尿来确定。我们前瞻性地检测了子痫前期和健康孕妇尿液中额外蛋白质（免疫球蛋白 G、转铁蛋白、α1-微球蛋白和β2-微球蛋白）的排泄模式。
对 1631 例连续妊娠妇女进行的前瞻性队列研究。
丹麦一家地区医院。
从队列中确定了 32 例子痫前期患者和 185 例健康对照妇女。尿液样本从第 18 周收集到分娩时，分为 6 个妊娠间隔。
用夹心 ELISA 法对尿液免疫球蛋白 G、转铁蛋白、α1-微球蛋白和β2-微球蛋白进行蛋白分析。
子痫前期和健康妊娠期间尿液中特定蛋白质的水平。
在妊娠 30 周后，子痫前期患者的免疫球蛋白 G 和转铁蛋白明显升高。α1-微球蛋白和β2-微球蛋白的排泄方式不同，在子痫前期中，在妊娠 36 周后升高，但只有α1-微球蛋白显著升高。
免疫球蛋白 G、转铁蛋白、α1-和β2-微球蛋白的排泄模式表明肾小球损伤，随后出现蛋白质的肾小管处理改变。
