Gorrec Fabrice, Palmer Colin M, Lebon Guillaume, Warne Tony
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB20QH, England.
Acta Crystallogr D Biol Crystallogr. 2011 May;67(Pt 5):463-70. doi: 10.1107/S0907444911008754. Epub 2011 Apr 7.
The Pi sampling method is derived from the incomplete factorial approach to macromolecular crystallization screen design. The resulting `Pi screens' have a modular distribution of a given set of up to 36 stock solutions. Maximally diverse conditions can be produced by taking into account the properties of the chemicals used in the formulation and the concentrations of the corresponding solutions. The Pi sampling method has been implemented in a web-based application that generates screen formulations and recipes. It is particularly adapted to screens consisting of 96 different conditions. The flexibility and efficiency of Pi sampling is demonstrated by the crystallization of soluble proteins and of an integral membrane-protein sample.
Pi抽样方法源自用于大分子结晶筛选设计的不完全析因法。由此产生的“Pi筛选”具有一组多达36种储备溶液的模块化分布。通过考虑配方中所用化学品的性质和相应溶液的浓度，可以产生最大限度多样化的条件。Pi抽样方法已在一个基于网络的应用程序中实现，该程序可生成筛选配方和方案。它特别适用于由96种不同条件组成的筛选。可溶性蛋白质和完整膜蛋白样品的结晶证明了Pi抽样的灵活性和效率。
