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Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
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HuR, a key post-transcriptional regulator, and its implication in progression of breast cancer.HuR，一种关键的转录后调控因子，及其在乳腺癌进展中的意义。
Histol Histopathol. 2010 Oct;25(10):1331-40. doi: 10.14670/HH-25.1331.
3
The x-ray crystal structure of the first RNA recognition motif and site-directed mutagenesis suggest a possible HuR redox sensing mechanism.第一个 RNA 识别基序的 X 射线晶体结构和定点突变提示了 HuR 氧化还原感应机制的可能性。
J Mol Biol. 2010 Apr 16;397(5):1231-44. doi: 10.1016/j.jmb.2010.02.043. Epub 2010 Feb 26.
4
Cloning, expression, purification and preliminary crystallographic studies of the adenylate/uridylate-rich element-binding protein HuR complexed with its target RNA.富含腺苷酸/尿苷酸元件结合蛋白HuR与其靶RNA复合物的克隆、表达、纯化及初步晶体学研究。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Mar 1;65(Pt 3):285-7. doi: 10.1107/S174430910900400X. Epub 2009 Feb 26.
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Diverse molecular functions of Hu proteins.Hu蛋白的多种分子功能。
Cell Mol Life Sci. 2008 Oct;65(20):3168-81. doi: 10.1007/s00018-008-8252-6.
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Lysine methylation as a routine rescue strategy for protein crystallization.赖氨酸甲基化作为蛋白质结晶的常规挽救策略。
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FEBS J. 2005 May;272(9):2118-31. doi: 10.1111/j.1742-4658.2005.04653.x.
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Identification of a target RNA motif for RNA-binding protein HuR.RNA结合蛋白HuR的靶RNA基序的鉴定
Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2987-92. doi: 10.1073/pnas.0306453101. Epub 2004 Feb 23.
9
Structural basis for recognition of AU-rich element RNA by the HuD protein.HuD蛋白识别富含AU元件RNA的结构基础。
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Structural basis for recognition of the tra mRNA precursor by the Sex-lethal protein.性致死蛋白识别tra mRNA前体的结构基础。
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HuR的RNA结合结构域的初步晶体学分析及其与聚尿苷的结合特性。

Preliminary crystallographic analysis of the RNA-binding domain of HuR and its poly(U)-binding properties.

作者信息

Wang Hong, Li Heng, Shi Hui, Liu Yang, Liu Huihui, Zhao Hui, Niu Liwen, Teng Maikun, Li Xu

机构信息

Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, People's Republic of China.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 May 1;67(Pt 5):546-50. doi: 10.1107/S1744309110052930. Epub 2011 Apr 21.

DOI:10.1107/S1744309110052930
PMID:21543858
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3087637/
Abstract

Human antigen R (HuR), a ubiquitously expressed member of the Hu protein family, is an important post-transcriptional regulator which has three RNA-recognition motif (RRM) domains. The two tandem N-terminal RRM domains can selectively bind to the AU-rich element (ARE), while the third one interacts with the poly(A) tail and other proteins. Here, the recombinant ARE-binding region of HuR (residues 18-186) was crystallized in space group P2(1)2(1)2, with unit-cell parameters a = 41.2, b = 133.1, c = 31.4 Å. X-ray diffraction data were collected to a resolution of 2.8 Å. Mutagenesis analysis and SPR assays revealed its poly(U)-binding properties.

摘要

人抗原R（HuR）是Hu蛋白家族中一种广泛表达的成员，是一种重要的转录后调节因子，具有三个RNA识别基序（RRM）结构域。两个串联的N端RRM结构域可以选择性地与富含AU元件（ARE）结合，而第三个结构域则与聚腺苷酸尾和其他蛋白质相互作用。在此，HuR的重组ARE结合区域（第18 - 186位氨基酸残基）在空间群P2(1)2(1)2中结晶，晶胞参数为a = 41.2，b = 133.1，c = 31.4 Å。X射线衍射数据收集到2.8 Å的分辨率。诱变分析和表面等离子体共振（SPR）测定揭示了其与聚尿苷酸（poly(U)）的结合特性。