Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life Science, South China Normal University, Guangzhou, China.
Plant Signal Behav. 2011 Jun;6(6):892-4. doi: 10.4161/psb.6.6.15317. Epub 2011 Jun 1.
UV-B radiation (280-315 nm) is an integral part of solar radiation and has many harmful effects on plant growth and development. However, the molecular mechanism for the inhibition of plant growth by UV-B remains largely unknown. UV-B radiation induces various responses such as growth inhibition, DNA damage and changes of gene expression. Recently, by using synchronous root tip culture, we found that UV-B modulates the expression of cell cycle regulatory genes through DNA damage. Western blotting analysis revealed that UV-B induced G1-to-S arrest did not correlate with the protein abundance of CDKB1;1 and CYCD3;1 gene regulating proteins, but may with the posttranslational control. We extended the expression analysis of cell cycle related genes based on the published microarray data and the results strengthen our assumption that cell cycle arrest could occur in plant under solar UV-B radiation. Further study is needed to elucidate the relationship between cell cycle regulation and protective pathway induced by low dose of UV-B radiation fundamental molecular mechanism for how plants respond to solar UV-B radiation.
UV-B 辐射（280-315nm）是太阳辐射的一个组成部分，对植物的生长和发育有许多有害影响。然而，UV-B 抑制植物生长的分子机制在很大程度上仍然未知。UV-B 辐射诱导各种反应，如生长抑制、DNA 损伤和基因表达的变化。最近，通过使用同步根尖培养，我们发现 UV-B 通过 DNA 损伤调节细胞周期调控基因的表达。Western blotting 分析表明，UV-B 诱导的 G1 期到 S 期阻滞与调节蛋白的 CDKB1;1 和 CYCD3;1 基因的蛋白丰度无关，而可能与翻译后调控有关。我们基于已发表的微阵列数据扩展了细胞周期相关基因的表达分析，结果增强了我们的假设，即在太阳 UV-B 辐射下，植物可能会发生细胞周期阻滞。需要进一步研究来阐明细胞周期调控与低剂量 UV-B 辐射诱导的保护途径之间的关系，这是植物如何响应太阳 UV-B 辐射的基本分子机制。
