Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, Omaha, Nebraska 68198-5840, USA.
Stem Cells. 2011 Jul;29(7):1013-20. doi: 10.1002/stem.655.
The reprogramming of somatic cells to a pluripotent state by the expression of a defined set of exogenous transcription factors represents a significant breakthrough for the use of stem cells in regenerative medicine. It has the potential to make autologous stem cell therapy practical and promote better understanding of the disease processes by generating patient specific stem cells. Several strategies have been used to generate induced pluripotent stem cells (iPSCs) that include nucleic acid and non-nucleic acid-based approaches, with and without epigenetic modifications. The purpose of these different approaches for generating iPSCs, besides understanding the underlying mechanism, is to develop a facile method for reprogramming without genetic alteration, suitable for clinical use. Here, we discuss different strategies for generating iPSCs, with an emphasis on a recent non-cell autonomous approach to reprogram somatic progenitors that regenerate cornea to a pluripotent state through the recruitment of endogenous transcription factors.
通过表达一组定义明确的外源性转录因子将体细胞重编程为多能状态，这代表了干细胞在再生医学中应用的重大突破。它有可能通过生成患者特异性干细胞使自体干细胞治疗变得切实可行，并促进对疾病过程的更好理解。已经使用了几种策略来生成诱导多能干细胞(iPSCs)，包括基于核酸和非核酸的方法，以及有和没有表观遗传修饰的方法。生成 iPSCs 的这些不同方法的目的，除了了解潜在机制之外，还在于开发一种无需遗传改变、适合临床应用的简便重编程方法。在这里，我们讨论了生成 iPSCs 的不同策略，重点介绍了最近的一种非细胞自主方法，该方法通过募集内源性转录因子将体细胞祖细胞重编程为多能状态，从而再生角膜。
