Food Protectants and Infestation Control Department, Central Food Technological Research Institute (CSIR Laboratory), Mysore 570020, India.
Anal Biochem. 2011 Aug 15;415(2):209-11. doi: 10.1016/j.ab.2011.04.014. Epub 2011 Apr 20.
This article describes a microplate-based kinetic assay for mitochondrial NADH-- and succinate--cytochrome c reductase activities in rat brain mitochondria. The assay reported here is based on the conventional spectrophotometric method and involves substrate-driven reduction of exogenous cytochrome c. Conditions regarding linearity with respect to time and protein concentration have been standardized. Furthermore, the methods were tested for inhibition of respective activities by specific inhibitors. The microplate format described here can be employed for rapid and simultaneous measurements of mitochondrial NADH-- and succinate--cytochrome c reductase activities in a large number of samples.
本文描述了一种基于微孔板的方法，用于测定大鼠脑线粒体中线粒体 NADH- 和琥珀酸-细胞色素 c 还原酶的活性。本报告中的测定方法基于传统的分光光度法，涉及外源性细胞色素 c 的底物驱动还原。已经标准化了线性时间和蛋白浓度的条件。此外，还测试了这些方法对特定抑制剂抑制各自活性的情况。这里描述的微孔板格式可用于快速和同时测量大量样品中线粒体 NADH- 和琥珀酸-细胞色素 c 还原酶的活性。
