Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy.
Mar Environ Res. 2011 Jun;71(5):351-6. doi: 10.1016/j.marenvres.2011.04.001. Epub 2011 Apr 15.
The effects of temperature on cellular and biochemical parameters of the crab Carcinus aestuarii were evaluated. Crabs were kept for 7 days at 4, 17 (reference value) and 30 °C (salinity of 35 psu), and total haemocyte count (THC), haemocyte volume, haemocyte proliferation, phenoloxidase (PO) activity in both haemocyte lysate (HL) and cell-free haemolymph (CFH), CFH total protein and glucose levels, superoxide dismutase (SOD) and catalase (CAT) activities in both gills and digestive gland were evaluated. The lowest and the highest temperature significantly decreased THC, whereas haemocyte volume and haemolymph glucose concentration did not differ significantly among experimental conditions. Haemolymph protein concentration significantly reduced in crabs maintained at 30 °C, when compared with that of animals kept at 4 and 17 °C. Haemocyte proliferation increased significantly in crabs kept at 4 and 30 °C, when compared with that of crabs held at 17 °C. Likewise, a significantly higher PO activity was recorded in CFH from crabs kept at 4 and 30 °C, than in control crabs. Conversely, PO activity did not vary significantly in HL. With regard to antioxidant enzyme activities, a significant decrease in CAT activity was observed in gills from crabs kept at 4 °C, when compared to that of crabs kept at 17 °C and 30 °C. Results obtained demonstrated that the highest and lowest temperature tested influenced crab biological responses, and indicated that C. aestuarii modulated its cellular and biochemical parameters (mainly haemocyte proliferation, CFH protein concentrations and CFH PO activity) in order to cope with temperature.
研究了温度对蟹 Carcinus aestuarii 细胞和生化参数的影响。将蟹在 4、17（参考值）和 30°C（盐度 35 psu）下分别饲养 7 天，并评估总血细胞计数（THC）、血细胞体积、血细胞增殖、血细胞溶酶体（HL）和无细胞血淋巴（CFH）中的酚氧化酶（PO）活性、CFH 总蛋白和葡萄糖水平、鳃和消化腺中的超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性。最低和最高温度显著降低了 THC，而血细胞体积和血淋巴葡萄糖浓度在实验条件之间没有显著差异。与在 4 和 17°C 下饲养的动物相比，在 30°C 下饲养的蟹的血淋巴蛋白浓度显著降低。与在 17°C 下饲养的蟹相比，在 4 和 30°C 下饲养的蟹的血细胞增殖显著增加。同样，与对照组相比，在 4 和 30°C 下饲养的蟹的 CFH 中的 PO 活性显著更高。相反，HL 中的 PO 活性没有显著变化。关于抗氧化酶活性，与在 17°C 下饲养的蟹相比，在 4°C 下饲养的蟹的鳃中的 CAT 活性显著降低。结果表明，测试的最高和最低温度影响了蟹的生物反应，并表明 C. aestuarii 调节了其细胞和生化参数（主要是血细胞增殖、CFH 蛋白浓度和 CFH PO 活性）以应对温度。
