Legan E, Sisson J A
Department of Internal Medicine, Texas Tech University Health Sciences Center, Lubbock 79430.
J Pharmacol Methods. 1990 Mar;23(1):31-9. doi: 10.1016/0160-5402(90)90006-7.
Arterial endothelium plays an essential role in modulating vascular tone through the production of vasoactive biogenic substances. To determine the effect of endothelial cell function on vascular smooth muscle receptor-effector coupling, a simple chemical techniques was devised to denude rat aortic endothelium while receptor function was preserved. Agonist-stimulated phosphatidylinositol turnover (PI) and vascular contractility were studied in saponin-treated aortic rings. Uniform removal of the aortic endothelial cell layer was readily verified with the use of a modified silver stain with light microscopy. Agonist-mediated PI turnover was statistically decreased in saponin-exposed aortic rings relative to control aorta. Increased contractile sensitivity in denuded aorta was also observed with norepinephrine (NE), phenylephrine (PE), and serotonin (5-HT). Thus, brief exposure to saponin is an effective chemical skinning technique that preserves vascular function.
动脉内皮通过产生血管活性生物物质在调节血管张力中起重要作用。为了确定内皮细胞功能对血管平滑肌受体 - 效应器偶联的影响，设计了一种简单的化学技术来剥脱大鼠主动脉内皮，同时保留受体功能。在皂角苷处理的主动脉环中研究了激动剂刺激的磷脂酰肌醇周转（PI）和血管收缩性。使用改良的银染和光学显微镜很容易证实主动脉内皮细胞层被均匀去除。与对照主动脉相比，皂角苷处理的主动脉环中激动剂介导的PI周转在统计学上降低。去内皮主动脉对去甲肾上腺素（NE）、苯肾上腺素（PE）和血清素（5 - HT）的收缩敏感性也增加。因此，短暂暴露于皂角苷是一种有效的化学去皮技术，可保留血管功能。
