Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
Vet Res Commun. 2011 Aug;35(6):329-36. doi: 10.1007/s11259-011-9476-8. Epub 2011 May 6.
Growth differentiation factor 9 (GDF9) was studied as a candidate gene for high prolificacy in goats. The polymorphism of exon 1 and flanking of GDF9 gene was detected by PCR-SSCP in five goat breeds with different prolificacy. Three genotypes (AA, AB and BB) were detected in goat breeds joined and two silent mutations (c.183A>C and c.336C>T) were identified in comparison genotype AA with genotype BB. Heterozygous genotype AB and wild type BB were detected in all five goat breeds and homozygous genotype AA was only detected in Jining Grey goats. The frequencies of genotypes AA, AB and BB were 0.18, 0.42 and 0.40 in Jining Grey goats, respectively. The genotype distribution was different (P < 0.01) between high prolificacy breed (Jining Grey goat) and low prolificacy breeds (Boer, Wendeng Dairy, Liaoning Cashmere and Beijing native goats). The Jining Grey goat does with genotype AA and AB had 0.72 (P < 0.01) and 0.56 (P < 0.01) kids more than those with genotype BB, respectively. The does with genotype AA had 0.16 (P > 0.05) kids more than those with genotype AB. These indicated that the allele A may have certain correlation with prolificacy in Jining Grey goats.
生长分化因子 9（GDF9）被研究为高产奶山羊的候选基因。采用 PCR-SSCP 方法对不同产羔数的 5 个山羊品种的 GDF9 基因外显子 1 及其侧翼区进行多态性检测，在这 5 个山羊品种中共检测到 3 种基因型（AA、AB 和 BB），与基因型 BB 相比，在基因型 AA 中发现了 2 个沉默突变（c.183A>C 和 c.336C>T）。在所有 5 个山羊品种中均检测到杂合基因型 AB 和野生型 BB，而仅在济宁青山羊中检测到纯合基因型 AA。在济宁青山羊中，AA、AB 和 BB 基因型的频率分别为 0.18、0.42 和 0.40。高产羔数品种（济宁青山羊）与低产羔数品种（波尔山羊、文登奶山羊、辽宁绒山羊和北京本地山羊）之间的基因型分布存在显著差异（P<0.01）。AA 和 AB 基因型的济宁青山羊母羊产羔数分别比 BB 基因型的母羊多 0.72（P<0.01）和 0.56（P<0.01）只。AA 基因型的母羊比 AB 基因型的母羊多产 0.16 只羔羊（P>0.05）。这些结果表明，A 等位基因可能与济宁青山羊的产羔数存在一定的相关性。
