Department of Zoology, University of Delhi, Delhi - 110007, India.
Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):618-623. doi: 10.1099/ijs.0.028886-0. Epub 2011 May 6.
A yellow-pigmented, hexachlorocyclohexane (HCH)-degrading bacterium, designated F2(T), was isolated from an HCH dumpsite at Ummari village in Lucknow, India. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus Sphingobium. Its closest neighbour was Sphingobium japonicum UT26(T) (99.2% 16S rRNA gene sequence similarity). The DNA G+C content was 65.7 mol%. The polyamine profile showed the presence of spermidine. The respiratory pigment was ubiquinone Q-10. The predominant cellular fatty acids were C(16:0) (12.5%), C(14:0) 2-OH (8.1%), summed feature 3 (consisting of C(16:1)ω7c and/or C(16:1)ω6c; 5.8%) and summed feature 8 (consisting of C(18:1)ω7c and/or C(18:1)ω6c; 53.1%). The major polar lipids of strain F2(T) were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and sphingoglycolipid. DNA-DNA relatedness and biochemical and physiological characters clearly distinguished the isolate from its closest phylogenetic neighbours. Thus, strain F2(T) represents a novel species of the genus Sphingobium, for which the name Sphingobium lucknowense sp. nov. is proposed. The type strain is strain F2(T) (=MTCC 9456(T) =CCM 7544(T)).
从印度勒克瑙的乌马里村的 HCH 倾倒场分离到一株黄色、六氯环己烷（HCH）降解菌，命名为 F2(T)。基于 16S rRNA 基因序列的系统发育分析表明，该分离物属于鞘氨醇单胞菌属。其最接近的亲缘种是日本鞘氨醇单胞菌 UT26(T)（99.2% 16S rRNA 基因序列相似性）。DNA G+C 含量为 65.7 mol%。多胺图谱显示存在亚精胺。呼吸色素为泛醌 Q-10。主要的细胞脂肪酸为 C(16:0)（12.5%）、C(14:0) 2-OH（8.1%）、特征总和 3（由 C(16:1)ω7c 和/或 C(16:1)ω6c 组成；5.8%）和特征总和 8（由 C(18:1)ω7c 和/或 C(18:1)ω6c 组成；53.1%）。菌株 F2(T) 的主要极性脂为磷脂酰乙醇胺、磷脂甲基乙醇胺、磷脂酰甘油、双磷脂酰甘油和神经酰胺糖脂。DNA-DNA 同源性以及生化和生理特征清楚地区分了该分离物与其最接近的系统发育近缘种。因此，菌株 F2(T) 代表了鞘氨醇单胞菌属的一个新种，建议命名为勒克瑙鞘氨醇单胞菌新种。模式菌株为 F2(T) 株（=MTCC 9456(T)=CCM 7544(T)）。
