Zhu Lingfang, Xin Kaiyun, Chen Chaoqiong, Li Changfu, Si Meiru, Zhao Liang, Shi Xu, Zhang Lei, Shen Xihui
State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
Antonie Van Leeuwenhoek. 2015 Apr;107(4):1001-8. doi: 10.1007/s10482-015-0392-8. Epub 2015 Jan 28.
A yellow-pigmented bacterium, designated strain GZGR-4(T), was isolated from the root of Hylomecon japonica (Thunb.) Prantl et Kündig collected from Taibai Mountain in Shaanxi Province, north-west China. Cells of strain GZGR-4(T) were Gram-negative, rod-shaped, strictly aerobic, non-endospore-forming and non-motile. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GZGR-4(T) is a member of the genus Sphingobium, exhibiting the highest sequence similarity to Sphingobium aromaticiconvertens DSM 12677(T) (97.3 %). 16S rRNA gene sequence similarities between strain GZGR-4(T) and the type strains of other Sphingobium species with validly published names ranged from 93.4-96.5 %. The predominant respiratory quinone of strain GZGR-4(T) was ubiquinone-10 (Q-10) and the major cellular fatty acids were summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c), summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), C16:0 and C14:0 2-OH. Spermidine was the major polyamine. The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid, one unidentified phosphoglycolipid, one unidentified phospholipid, one unidentified aminolipid and one unidentified lipid. The DNA G+C content was 63.6 mol%. DNA-DNA relatedness for strain GZGR-4(T) with respect to its closest phylogenetic relative S. aromaticiconvertens DSM 12677(T) was 22.6 ± 5.3 %. On the basis of the polyphasic taxonomic data presented, strain GZGR-4(T) is considered to represent a novel species of the genus Sphingobium, for which the name Sphingobium endophyticus sp. nov. is proposed. The type strain is GZGR-4(T) (=CCTCC AB 2013305(T) = KCTC 32447(T)).
从中国西北部陕西省太白山采集的日本荷青花（Hylomecon japonica (Thunb.) Prantl et Kündig）根部分离出一株黄色色素细菌，命名为菌株GZGR-4(T)。菌株GZGR-4(T)的细胞为革兰氏阴性、杆状、严格需氧、不形成芽孢且不运动。基于16S rRNA基因序列的系统发育分析表明，菌株GZGR-4(T)是鞘氨醇单胞菌属（Sphingobium）的成员，与芳香鞘氨醇单胞菌（Sphingobium aromaticiconvertens）DSM 12677(T)的序列相似性最高（97.3%）。菌株GZGR-4(T)与其他已有效发表名称的鞘氨醇单胞菌属模式菌株之间的16S rRNA基因序列相似性范围为93.4 - 96.5%。菌株GZGR-4(T)的主要呼吸醌为泛醌-10（Q-10），主要细胞脂肪酸为总和特征8（包含C18:1 ω7c和/或C18:1 ω6c）、总和特征3（包含C16:1 ω7c和/或C16:1 ω6c）、C16:0和C14:0 2-OH。亚精胺是主要的多胺。极性脂质谱由磷脂酰甘油、二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、鞘糖脂、一种未鉴定的磷酸糖脂、一种未鉴定的磷脂、一种未鉴定的氨基脂和一种未鉴定的脂类组成。DNA G+C含量为63.6 mol%。菌株GZGR-4(T)与其最接近的系统发育亲缘种芳香鞘氨醇单胞菌DSM 12677(T)的DNA-DNA相关性为22.6 ± 5.3%。基于所提供的多相分类数据，菌株GZGR-4(T)被认为代表鞘氨醇单胞菌属的一个新物种，为此提出名为内生鞘氨醇单胞菌（Sphingobium endophyticus）的新种。模式菌株为GZGR-4(T)（=CCTCC AB 2013305(T) = KCTC 32447(T)）。
