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眼表和泪器中抗菌肽 psoriasin(S100A7)的表达和调节。

Expression and regulation of antimicrobial peptide psoriasin (S100A7) at the ocular surface and in the lacrimal apparatus.

机构信息

Department of Anatomy II, Friedrich Alexander University Erlangen-Nuremberg, Erlangen, Germany.

出版信息

Invest Ophthalmol Vis Sci. 2011 Jul 1;52(7):4914-22. doi: 10.1167/iovs.10-6598.

Abstract

PURPOSE

Psoriasin, originally isolated from psoriasis as an overexpressed molecule of unknown function, has recently been identified as a principal Escherichia coli-killing antimicrobial peptide of healthy skin. The purpose of this study was to investigate the expression and antimicrobial role of psoriasin at the ocular surface and in the lacrimal apparatus.

METHODS

Different tissues of the lacrimal apparatus and ocular surface were systematically analyzed by means of RT-PCR, Western blot, and immunohistochemistry for their ability to express and produce psoriasin. The inducibility and regulation of psoriasin were studied in human corneal as well as conjunctival epithelial cell lines after challenge with ocular pathogens and proinflammatory cytokines. Real-time RT-PCR was performed to evaluate the expression and induction of psoriasin. In addition, tear fluid obtained from different healthy volunteers was examined by ELISA for its psoriasin concentration.

RESULTS

RT-PCR and Western blot analyses revealed a constitutive expression of psoriasin in cornea, conjunctiva, nasolacrimal ducts, and lacrimal gland. Immunohistochemistry showed strong staining of meibomian glands for psoriasin. No induction of psoriasin was observed after stimulation with supernatants of E. coli, whereas supernatants of Staphylococcus aureus and Haemophilus influenzae significantly increased the psoriasin mRNA expression. Stimulation with IL-1β and VEGF also strongly increased psoriasin transcription. The highest amounts of psoriasin protein were detected in the tear fluid (~170 ng/mL) of healthy volunteers.

CONCLUSIONS

The results suggest that psoriasin is produced by the structures of the ocular surface and is part of the innate immune system at the ocular surface and tear film.

摘要

目的

Psoriasin 最初从银屑病中分离出来,是一种功能未知的过度表达分子,最近被鉴定为健康皮肤中主要的大肠杆菌杀伤抗菌肽。本研究旨在研究 psoriasin 在眼表面和泪器中的表达和抗菌作用。

方法

通过 RT-PCR、Western blot 和免疫组织化学分析,对泪器和眼表面的不同组织进行了系统分析,以研究其表达和产生 psoriasin 的能力。在人角膜和结膜上皮细胞系中,用眼部病原体和促炎细胞因子刺激后,研究了 psoriasin 的诱导和调节。通过实时 RT-PCR 评估 psoriasin 的表达和诱导。此外,通过 ELISA 检测来自不同健康志愿者的泪液中 psoriasin 的浓度。

结果

RT-PCR 和 Western blot 分析显示,角膜、结膜、鼻泪管和泪腺中存在 psoriasin 的组成型表达。免疫组织化学显示,meibomian 腺对 psoriasin 染色强烈。用大肠杆菌上清液刺激后,未观察到 psoriasin 的诱导,而金黄色葡萄球菌和流感嗜血杆菌的上清液显著增加了 psoriasin mRNA 的表达。IL-1β 和 VEGF 的刺激也强烈增加了 psoriasin 的转录。在健康志愿者的泪液中检测到最高量的 psoriasin 蛋白(~170ng/ml)。

结论

这些结果表明,psoriasin 由眼表面结构产生,是眼表面和泪膜固有免疫系统的一部分。

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