Department of Neuroscience, Karolinska Institute, Doktorsringen 17, S-171 77 Stockholm, Sweden Department of Surgical Sciences, Section of Rehabilitation Medicine, Karolinska Institute, Karolinska Hospital, Stockholm, Sweden.
Restor Neurol Neurosci. 1996 Jan 1;10(3):123-33. doi: 10.3233/RNN-1996-10301.
Isolectin B4 from Griffonia simplicifolia I (B4) has a high binding affinity to a large population of unmyelinated primary sensory neurons (Wang et al., Neuroscience 62 (1994) 539-551). Using immunohistochemical techniques, binding and transganglionic transport of B4 in the spinal cord was investigated, both at short and long survival times, after sciatic nerve transection and ligation or crush in the adult rat. Nerve transection and ligation resulted in nearly complete disappearance of B4 immunolabelling in the sciatic nerve territory of the superficial dorsal horn after B4 binding, as well as after transganglionic transport of B4 by 2 weeks postinjury. Partial recovery of both B4 binding and B4 transport was found by 8 months, and nearly complete recovery by 16 months, indicating that reappearance of B4 binding is not critically dependent on peripheral reinnervation. Crush injury made by jeweller's forceps resulted in partial depletion of binding and transport by 2 weeks and a nearly complete recovery by 10 weeks. The results show that binding and transganglionic transport of B4 can be used to label dorsal horn connections of unmyelinated primary afferents during the process of regeneration after crush injury. Furthermore, as B4 binding and transport recover at long survival times in the absence of reestablished peripheral connections, the same techniques can be used to study central primary afferent connections at long survival times after nerve transection. Binding and transganglionic transport of B4 offer alternatives to the use of previous techniques such as transganglionic transport of wheat germ agglutinin conjugated horseradish peroxidase (WGA-HRP) to study central connections of fine primary afferents after injury.
相思豆凝集素 B4 来自 Griffonia simplicifolia I（B4），对大量未髓鞘化的初级感觉神经元具有高亲和力（Wang 等人，神经科学 62（1994）539-551）。使用免疫组织化学技术，在成年大鼠坐骨神经横断和结扎或挤压后，研究了 B4 在脊髓中的结合和跨节转运，包括短期和长期存活时间。神经横断和结扎导致 B4 结合后，坐骨神经区域的浅层背角中几乎完全消失了 B4 免疫标记，以及 B4 通过损伤后 2 周的跨节转运。在 8 个月时发现 B4 结合和 B4 转运都有部分恢复，在 16 个月时几乎完全恢复，表明 B4 结合的重新出现并不严重依赖于周围的再神经支配。珠宝镊子造成的挤压损伤在 2 周时导致结合和转运部分耗竭，在 10 周时几乎完全恢复。结果表明，在挤压损伤后的再生过程中，B4 的结合和跨节转运可用于标记未髓鞘化初级传入纤维的背角连接。此外，由于 B4 结合和转运在没有重建的周围连接的情况下在长时间存活时恢复，因此相同的技术可用于研究神经横断后长时间存活时的中枢初级传入连接。B4 的结合和跨节转运为以前的技术提供了替代方法，例如用结合辣根过氧化物酶的麦胚凝集素（WGA-HRP）进行跨节转运，以研究损伤后精细初级传入纤维的中枢连接。
