Department of Experimental Psychology, University of Cambridge, Cambridge (U.K.).
Restor Neurol Neurosci. 1989 Jan 1;1(1):83-92. doi: 10.3233/RNN-1989-1109.
Wound-derived trophic factors released by the injured brain are thought to reach a peak 1-2 weeks after injury. It has been proposed that such factors can promote the survival, growth and functional capacity of embryonic tissue grafts. To test the generality of this hypothesis, control rats and rats with aspirative fimbria-fornix lesions were compared with 5 groups of rats with lesions and septal grafts implanted either in the same session as the lesion or after delays from 10 days to 9 months. Animals were assessed 3 months post-transplantation on an operant differential reinforcement of low rates (DRL) task and on a test of spontaneous locomotor activity. Lesions produced impairments on all measures of DRL performance. Two graft groups showed amelioration of the DRL deficits, one graft group was unchanged, and the deficits were exacerbated in two others. There was no clear relationship between lesion-graft interval and recovery. An inverse relationship was seen, however, between recovery and the developmental age of the donor tissue. In contrast, significant recovery from lesion-induced hyperactivity was observed in the two graft groups with tissue derived from the oldest embryos. There were no clear relationships between recovery on either test, lesion-graft interval, and AChE-positive reinnervation of the host brain. The results provide further evidence that septal grafts can reverse behavioural deficits induced by fimbria-fornix lesions under some conditions, but suggest that the timing of graft surgery may not be as important a factor as donor age in this model system.
伤口衍生的营养因子由受伤的大脑释放，据认为其在损伤后 1-2 周达到高峰。有人提出，这些因子可以促进胚胎组织移植物的存活、生长和功能能力。为了检验这一假说的普遍性，将对照大鼠和横切穹窿海马伞损伤大鼠与 5 组损伤和隔核移植物大鼠进行比较，这些大鼠的损伤和隔核移植物分别在损伤的同时或在 10 天至 9 个月的延迟后植入。在移植后 3 个月，动物接受操作性条件性低频区分性强化（DRL）任务和自发运动活动测试。损伤导致 DRL 表现的所有指标受损。两个移植物组改善了 DRL 缺陷，一个移植物组不变，另外两个移植物组的缺陷加重。损伤-移植物间隔与恢复之间没有明确的关系。然而，在恢复与供体组织的发育年龄之间观察到了相反的关系。相比之下，在来自最老胚胎的组织的两个移植物组中观察到了对损伤诱导的过度活跃的显著恢复。在这两种测试中的任何一种、损伤-移植物间隔和宿主大脑的 AChE 阳性再支配之间，都没有明显的关系。这些结果进一步证明，在某些条件下，隔核移植物可以逆转穹窿海马伞损伤引起的行为缺陷，但表明在这个模型系统中，移植物手术的时机可能不如供体年龄重要。
