CNS Growth Factor Research Laboratory, Massachusetts General Hospital, Boston, MA 02214 (U.S.A.) Department of Neurology, Harvard Medical School, Boston, MA 02115, (U.S.A.).
Restor Neurol Neurosci. 1990 Jan 1;1(6):387-94. doi: 10.3233/RNN-1990-1603.
Focal injury to the mammalian central nervous system (CNS) results in a cascade of cellular responses - including glial and capillary proliferation and neural sprouting - that contribute to the repair of neural tissue and to the recovery of neurological function. Fibroblast growth factors (FGFs) are heparin-binding polypeptides with potent trophic effects on CNS glia, endothelia, and neurons; both acidic and basic forms are found in the mammalian CNS. We used heparin-affinity chromatography coupled to Balb/c 3T3 mitogenic assay to show a marked increase in levels of bioactive FGFs in tissue surrounding focal cortical lesions of the mature rat brain at one week after injury. Heparin-affinity HPLC showed that this increase was due to a large increase in levels of basic FGF (bFGF), and a much smaller increase in levels of acidic FGF (aFGF) after injury. Increased bFGF bioactivity was paralleled by increased levels of immunoreactive bFGF, as assessed by Western blotting techniques. Increased bFGF levels may play an important role in the cascade of cellular reactions occurring after focal brain injury.
哺乳动物中枢神经系统 (CNS) 的局灶性损伤会引发一系列细胞反应 - 包括神经胶质和毛细血管增生以及神经发芽 - 这些反应有助于修复神经组织并恢复神经功能。成纤维细胞生长因子 (FGFs) 是一种肝素结合的多肽，对 CNS 神经胶质、内皮细胞和神经元具有很强的营养作用；酸性和碱性形式都存在于哺乳动物的 CNS 中。我们使用肝素亲和层析结合 Balb/c 3T3 有丝分裂测定法，显示在成熟大鼠脑局灶性皮质损伤后一周，损伤周围组织中生物活性 FGFs 的水平显著增加。肝素亲和 HPLC 显示，这种增加是由于碱性 FGF (bFGF) 水平的大幅增加，以及损伤后酸性 FGF (aFGF) 水平的小幅度增加。生物活性 bFGF 的增加与免疫反应性 bFGF 水平的增加平行，如通过 Western 印迹技术评估的那样。增加的 bFGF 水平可能在局灶性脑损伤后发生的细胞反应级联中发挥重要作用。
