Department of Medical Cell Research, University of Lund, Lund (Sweden) Department of Medical Physiology, University of Munich, Munich (F.R.G.).
Restor Neurol Neurosci. 1991 Jan 1;2(3):123-35. doi: 10.3233/RNN-1991-2302.
Ongoing clinical trials with fetal tissue transplants in Parkinson's disease would be facilitated by an effective tissue storage technique that would allow for temporal separation of the procurement of the fetal donor tissue and implantation surgery. In order to develop such a method, we grafted rat or human fetal ventral mesencephalic tissue to the dopamine-depleted striatum of rats either directly, or following pregraft refrigeration in a 'hibernation' medium at 4 °C. Rat tissue transplants were found to normalize amphetamine-induced circling behavior at 6 weeks post-transplantation after having been hibernated for either 2 or 5 days. The number of tyrosine hydroxylase immunoreactive neurons in these hibernated grafts did not differ significantly from that found in matched grafts of fresh tissue. Hibernation for 10 days resulted both in an absence of functional effects and in decreases of graft survival down to 10-20% of control values. Volume assessment of fresh and hibernated grafts prepared from human fetal tissue revealed no adverse effects of a 3 day hibernation interval at 3 weeks after transplantation into immunosuppressed rats. The results indicate that hibernation of neural tissue may be a convenient and simple tool, which can help to guarantee tissue availability at the planned time of implantation in patients and facilitate transport and bacteriological examination. Furthermore, the method offers a simple means which permits prolonged exposure of the neural tissue to trophic factors and specific markers prior to grafting in experimental animals.
正在进行的用胎儿组织移植治疗帕金森病的临床试验将得益于一种有效的组织储存技术，该技术可以使胎儿供体组织的获取和移植手术在时间上分开。为了开发这样一种方法，我们将大鼠或人胎儿腹侧中脑组织直接移植到多巴胺耗竭的大鼠纹状体中，或者在 4°C 的“冬眠”介质中预移植冷藏后再移植。研究发现，大鼠组织移植在冷藏 2 天或 5 天后进行冬眠，可在移植后 6 周时使安非他命诱导的转圈行为正常化。这些冬眠移植物中的酪氨酸羟化酶免疫反应性神经元数量与新鲜组织的匹配移植物没有显著差异。冬眠 10 天不仅导致功能效应缺失，而且使移植物存活率下降到对照值的 10-20%。从人胎儿组织制备的新鲜和冬眠移植物的体积评估显示，在免疫抑制大鼠中移植 3 周后，3 天的冬眠间隔没有不良影响。结果表明，神经组织的冬眠可能是一种方便和简单的工具，可以帮助保证在计划的植入时间为患者提供组织，并便于运输和细菌学检查。此外，该方法提供了一种简单的方法，允许在实验动物中移植前将神经组织长时间暴露于营养因子和特定标志物中。
