Rodriguez Alba I, Willing Alison E, Saporta Samuel, Cameron Don F, Sanberg Paul R
Center of Excellence for Aging and Brain Repair, Department of Neurosurgery, The Neuroscience Program, College of Medicine, University of South Florida, Tampa, FL 33612, USA.
Neurotox Res. 2003;5(6):443-50. doi: 10.1007/BF03033174.
Problems with immunosuppression and graft survival limit clinical applications of neurotransplantation protocols for neurodegenerative disease. Sertoli cells, testes-derived cells with immunosuppressive and trophic properties, may serve as an alternative cell source for transplantation. Sertoli cells were transplanted into the striatum of rats following two injections of 3-nitropropionic acid (3-NP) to determine whether they could ameliorate abnormalities in a model of early stage Huntington's disease. 3-NP-induced locomotor hyperactivity was significantly reduced in rats receiving Sertoli transplants compared to controls, with some behaviors returning to baseline. Sertoli cells survived in the striatum without systemic immunosuppression and some formed tubule-like structures. These results show that Sertoli transplants are able to ameliorate locomotor abnormalities in a 3-NP model of early HD. Thus, Sertoli cells should be further evaluated as a possible treatment strategy for the early stages of Huntington's disease.
免疫抑制和移植物存活问题限制了神经移植方案在神经退行性疾病临床应用中的发展。支持细胞是源自睾丸的具有免疫抑制和营养特性的细胞，可作为移植的替代细胞来源。在给大鼠注射两次3-硝基丙酸（3-NP）后，将支持细胞移植到大鼠纹状体中，以确定它们是否能改善早期亨廷顿舞蹈症模型中的异常情况。与对照组相比，接受支持细胞移植的大鼠中，3-NP诱导的运动亢进明显减少，一些行为恢复到基线水平。支持细胞在没有全身免疫抑制的情况下在纹状体中存活，并且一些形成了管状结构。这些结果表明，支持细胞移植能够改善早期亨廷顿舞蹈症3-NP模型中的运动异常。因此，支持细胞应作为亨廷顿舞蹈症早期可能的治疗策略进行进一步评估。
