Research Center of the Proyecto Camina A.C. Tlalpan 4430, C.P. 14050 Mexico City, Mexico Department of Clinical Research in Neurological Diseases, Hospital de Especialidades, Centro Medico Nacional Siglo XXI, Inslituto Mexicano del Seguro Social, Mexico City, Mexico.
Restor Neurol Neurosci. 1994 Jan 1;7(2):61-70. doi: 10.3233/RNN-1994-7201.
To evaluate the neuroprotective effect of transplants placed in the lesion zone after a complete spinal cord (SC) laceration, two independent series of experiments were carried out. In the first, allogeneic or xenogeneic fetal SC was transplanted into the gaps of the damaged lower thoracic SC of adult rats. In the transplanted rats the incidence of life-threatening complications was reduced, and the survival rate was increased compared with the control group (lesion, without implant). Histological examination showed less damage to the neighboring SC parenchyma in the transplanted rats. The measurement of this neuroprotective effect was made in a second series of experiments. Using the same model of SC injury, allogeneic fetal SC, autologous peripheral nerve and/or adipose tissue were implanted. Rats with implants of Gelfoam and damaged rats without implants were the controls. The implanted rats of all groups, including the Gelfoam group, showed a better survival rate than the nonimplanted rats. Significantly less damage to the neighboring SC parenchyma was measured in implanted rats with any of the live tissues tested compared with non-implanted rats, although no significant differences were observed between the Gelfoam group and the nonimplanted rats. Histological evidence of tissue implant survival was observed in all corresponding groups. It is concluded that the transplanted tissues tested here have a neuroprotective effect, possibly by acting as a buffer to neurotoxic substance(s) released by the stumps, and/or by exerting trophic effect(s) on the host.
为了评估完全性脊髓横断伤后移植到损伤部位的移植物的神经保护作用，我们进行了两项独立的实验系列。在第一项实验中，将同种异体或异种胎儿脊髓移植到成年大鼠损伤的下胸段脊髓的间隙中。与对照组（损伤，无植入物）相比，移植大鼠的危及生命并发症发生率降低，存活率提高。组织学检查显示，移植大鼠相邻脊髓实质的损伤较小。在第二项实验系列中，使用相同的脊髓损伤模型，植入同种异体胎儿脊髓、自体周围神经和/或脂肪组织。使用 Gelfoam 植入物的大鼠和未植入物的损伤大鼠作为对照。包括 Gelfoam 组在内的所有植入组的大鼠的存活率均高于未植入组的大鼠。与未植入组大鼠相比，植入任何活组织的大鼠相邻脊髓实质的损伤明显减少，但 Gelfoam 组与未植入组大鼠之间无显著差异。在所有相应的实验组中都观察到组织植入物的存活。因此，可以得出结论，这里测试的移植组织具有神经保护作用，可能通过充当由残端释放的神经毒性物质的缓冲剂，和/或对宿主发挥营养作用而起作用。
