Lightfoot K, Tai A, Segev O
Int J Oncol. 1995 Nov;7(5):1185-91. doi: 10.3892/ijo.7.5.1185.
The Drosophila ras2 and rop gene pair are governed by a common promoter. Characterisation of the Drosophila ras2/rop bidirectional promoter has revealed that a single major protein complex (M), composed of two subunits (factors A and B) interacts with two central promoter domains (regions A and B). To define the transcriptional control elements contained within region A (-58 to -39) we performed mutational analysis on several putative elements. This approach has revealed that the conserved TATA-like, SRE-like and GATA-like sequences do not interact with factor A and are not regulatory elements of this promoter. Nucleotides -56 to -49 bear perfect homology to the Drosophila DNA replication-related element (DRE, TATCGATA) found in two genes involved in DNA replication and cell proliferation, the Drosophila proliferating cell nuclear antigen (PCNA) and DNA polymerase a. A CG pair (-53 and -52) has been pinpointed as cardinal for factor A binding. Factor A has been partially purified and identified as a 20 kDa polypeptide by photo-chemical crosslinking analysis.
果蝇ras2和rop基因对受一个共同启动子调控。对果蝇ras2/rop双向启动子的特性分析表明，由两个亚基（因子A和因子B）组成的单一主要蛋白复合物（M）与两个中央启动子结构域（区域A和区域B）相互作用。为了确定区域A（-58至-39）中所含的转录控制元件，我们对几个假定元件进行了突变分析。该方法表明，保守的类TATA、类SRE和类GATA序列不与因子A相互作用，不是该启动子的调控元件。核苷酸-56至-49与在参与DNA复制和细胞增殖的两个基因（果蝇增殖细胞核抗原（PCNA）和DNA聚合酶α）中发现的果蝇DNA复制相关元件（DRE，TATCGATA）具有完全同源性。一个CG对（-53和-52）已被确定为因子A结合的关键位点。通过光化学交联分析，因子A已被部分纯化并鉴定为一种20 kDa的多肽。
