Qiao Xianli, Jiang Shuguang, Li Xiaofeng, Li Fengxia, Zhao Dexiu
Key Laboratory for Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Sheng Wu Gong Cheng Xue Bao. 2011 Jan;27(1):69-75.
We investigated the plant regeneration and production of flavonoids in three high-yield flavonoids transgenic Saussurea involucrata hairy roots C17, C27 and C46 by quantification of two phytohormones GA3 and IAA. The results showed that GA3 concentration at more than 1.0 mg/L could induce adventitious shoots in the hairy root lines. The highest shoot regeneration rate, about 82%, was obtained when the hairy roots C17 were cultured with 2.0 mg/L GA3. The results on HPLC and UV spectrophotometry showed that exogenous application of both GA3 and IAA increased the content of flavonoids in the hairy roots. The contents of flavonoids and apigenin in the hormone-treated hairy roots and regenerates were higher comparing with those in the untreated hairy roots and the regenerates. However, the content of flavonoids was not related to tissue weight, and was negatively related to the regeneration efficiency.
我们通过对两种植物激素赤霉素（GA3）和吲哚乙酸（IAA）进行定量分析，研究了三种高产黄酮类化合物的转基因雪莲毛状根C17、C27和C46中的植株再生及黄酮类化合物的生成情况。结果表明，GA3浓度超过1.0 mg/L时可诱导毛状根系产生不定芽。当毛状根C17用2.0 mg/L GA3培养时，获得了最高的芽再生率，约为82%。高效液相色谱法（HPLC）和紫外分光光度法的结果表明，外源施加GA3和IAA均增加了毛状根中黄酮类化合物的含量。与未处理的毛状根和再生植株相比，激素处理的毛状根和再生植株中黄酮类化合物和芹菜素的含量更高。然而，黄酮类化合物的含量与组织重量无关，且与再生效率呈负相关。
