[Effects of phytohormones on hairy root growth and tanshinone biosynthesis of Salvia miltiorrhiza].

OBJECTIVE

To study the effects of different phytohormones on the growth of hairy root and biosynthesis of tanshinone II A in Salvia miltiorrhiza.

METHODS

Salvia miltiorrhiza hairy root was induced by organizational culture method. Tanshinone was extracted with ultrasonic method and detected by high performance liquid chromatography (HPLC).

RESULTS

The phytohormone combination of GA3 and 6-BA at different concentrations adding to 1/2 MS medium had obviously inhibitory effect on the hairy root growth of Salvia miltiorrhiza. And the highest inhibition rate was up to 100% (hairy roots were death). However, the growth of hairy roots could be obviously promoted by the combination of KT (1.0 mg/L) and IBA (1.0 mg/L), increasing 3. 251 times than that of the control. Total tanshinone II A content in hairy roots bodies was the highest under the combination of 0.2 mg/L NAA and 3.0 mg/L 6-BA. 6-BA of 2.0 mg/L played a significant role in promoting tanshinone II A biosynthesis, and the tanshinone II A concentration was 3.012 times higher than that of the control.

CONCLUSION

Different phytohormones and its combination adding to 1/2 MS medium has significant influence on the hairy root growth and the content of tanshinone II A in Salvia miltiorrhiza.