Järvenpää P
Department of Clinical Chemistry, University of Helsinki, Meilahti Hospital, Finland.
J Steroid Biochem. 1990 Feb;35(2):289-92. doi: 10.1016/0022-4731(90)90286-2.
Intestinal bacterial metabolism of the catechol estrogens, 2-hydroxyestrone (2-OHE1), 2-methoxyestrone (2-MeOE1) and 2-hydroxyestradiol (2-OHE2), was studied by incubation of the steroid with mixed fecal flora. 3-Methoxyestrone (3-MeOE1) was included in order to evaluate whether demethylation occurs also with regard to noncatecholic estrogens. The purification of the samples and separation into fractions was carried out by ion exchange chromatography and the metabolites formed were identified by combined gas chromatography-mass spectrometry (GC-MS). Mixed human fecal flora was able to interconvert 2-OHE1 and 2-OHE2 both in aerobic and anaerobic conditions. Demethylation occurred both for 2-MeOE1 and 3-MeOE1.
通过将儿茶酚雌激素、2-羟基雌酮（2-OHE1）、2-甲氧基雌酮（2-MeOE1）和2-羟基雌二醇（2-OHE2）与混合粪便菌群一起孵育，研究了它们在肠道中的细菌代谢情况。加入3-甲氧基雌酮（3-MeOE1）是为了评估非儿茶酚雌激素是否也会发生去甲基化。通过离子交换色谱法对样品进行纯化并分离成不同组分，利用气相色谱-质谱联用（GC-MS）对形成的代谢产物进行鉴定。人类混合粪便菌群在需氧和厌氧条件下均能使2-OHE1和2-OHE2相互转化。2-MeOE1和3-MeOE1均发生了去甲基化。
