CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
BMC Genomics. 2011 May 10;12:219. doi: 10.1186/1471-2164-12-219.
Our previous studies revealed that a new disease form of streptococcal toxic shock syndrome (STSS) is associated with specific Streptococcus suis serotype 2 (SS2) strains. To achieve a better understanding of the pathogenicity and evolution of SS2 at the whole-genome level, comparative genomic analysis of 18 SS2 strains, selected on the basis of virulence and geographic origin, was performed using NimbleGen tiling arrays.
Our results demonstrate that SS2 isolates have highly divergent genomes. The 89K pathogenicity island (PAI), which has been previously recognized as unique to the Chinese epidemic strains causing STSS, was partially included in some other virulent and avirulent strains. The ABC-type transport systems, encoded by 89K, were hypothesized to greatly contribute to the catastrophic features of STSS. Moreover, we identified many polymorphisms in genes encoding candidate or known virulence factors, such as PlcR, lipase, sortases, the pilus-associated proteins, and the response regulator RevS and CtsR. On the basis of analysis of regions of differences (RDs) across the entire genome for the 18 selected SS2 strains, a model of microevolution for these strains is proposed, which provides clues into Streptococcus pathogenicity and evolution.
Our deep comparative genomic analysis of the 89K PAI present in the genome of SS2 strains revealed details into how some virulent strains acquired genes that may contribute to STSS, which may lead to better environmental monitoring of epidemic SS2 strains.
我们之前的研究表明，一种新型的链球菌中毒性休克综合征（STSS）疾病形式与特定的猪链球菌 2 型（SS2）菌株有关。为了更好地了解 SS2 在全基因组水平上的致病性和进化，我们使用 NimbleGen 平铺阵列对基于毒力和地理起源选择的 18 株 SS2 菌株进行了比较基因组分析。
我们的结果表明，SS2 分离株具有高度分化的基因组。先前被认为是引起 STSS 的中国流行株特有的 89K 致病岛（PAI）部分包含在一些其他毒力和非毒力菌株中。由 89K 编码的 ABC 型转运系统被假设对 STSS 的灾难性特征有很大贡献。此外，我们在编码候选或已知毒力因子的基因中发现了许多多态性，如 PlcR、脂肪酶、分选酶、菌毛相关蛋白以及应答调节子 RevS 和 CtsR。基于对 18 株选定的 SS2 菌株整个基因组差异区（RD）的分析，提出了这些菌株的微进化模型，为链球菌的致病性和进化提供了线索。
我们对 SS2 菌株基因组中 89K PAI 的深入比较基因组分析揭示了一些毒力菌株如何获得可能导致 STSS 的基因的细节，这可能有助于更好地对流行的 SS2 菌株进行环境监测。
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